SHK-1
97111106, fish unknown/unspecified, Fibroblast-like
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About This Item
UNSPSC Code:
41106514
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product name
SHK-1, 97111106
biological source
fish unknown/unspecified
growth mode
Adherent
karyotype
Not specified
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Atlantic salmon head kidney
Cell Line Description
The cell line was established from primary cultures of adherent cells from Atlantic salmon head kidney. The cells have been used in the study of the infectious salmon anaemia (ISA) virus. SHK-1 cells are recognised by MAbs specific for Atlantic salmon peripheral blood leucocytes, but not with Mabs specific with polymorphonuclear leucocytes. SHK-1 cells have been shown to phagocytose the fish pathogen Aeromonas salmonicidia to some extent, but no bacterical activity was observed over a period of 72 hours. From these results it has been concluded that SHK-1 is derived from leucocytes with some of the properties of macrophages. Studies have shown that the cells can be passaged more than 60 times.
Application
Propagation of infectious salmon anaemia (ISA) virus
SHK-1 cell line has been used to determine the effects of different nucleotide concentrations on cell viability. It has also been used to study the effects of Silymarin, a compound used in aquaculture.
Culture Medium
Leibovitz L15 + 4mM Glutamine + 40μM 2-Me + 5% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:2 seeding at 5 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA. Incubate at 20°C. CO2 must not be used. Keep culture dark e.g. by wrapping flask in foil. The cells are sensitive to trypsin which must be removed at each subculture by centrifuging the harvested cells at 100 x g for 5 minutes and resuspending the cell pellet in fresh medium. Cells grow better if conditioned medium is used at each stage supplemented with fresh medium.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Birgit H Dannevig, National Veterinary Institute, Ullevaalsveien 68; Oslo
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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