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biological source
Mouse x Rat Hybridoma nerve
growth mode
Adherent
karyotype
Not specified
morphology
Neuronal
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse neuroblastoma x Rat neurone hybrid
Cell Line Description
The hybrid cell line ND15 was derived after fusion of primary neonatal-rat dorsal-root-ganglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300)- derived azaguanine-resistant line. HAT-sensitive hybrids were selected and cloned by limiting dilution. The cells express all isoforms of N-CAM but no embryonic form. Thy 1.1 marker, P31 and PrPc can be detected as well as a weaker expression of F3 and Thy-3. When differentiated in serum free media an increased level of Thy-2 can be detected.
Application
Study of neuronal differentiation, cell adhesion and expression of neuronal proteins
Culture Medium
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 by either aspirating or gently tapping the cells loose; 5% CO2; 37°C. Cells are lightly attached therefore use of trypsin is unnecessary.
Other Notes
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Certificates of Analysis (COA)
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