Skip to Content
Merck
All Photos(2)

Key Documents

View All Documentation

CASP3F

Sigma-Aldrich

Caspase 3 Assay Kit, Fluorimetric

Synonym(s):

Caspase-3 Activity Fluorometric Kit

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

EC Number:
247-454-8
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 1,000 multiwell tests
 sufficient for 100 standard tests (2 ml)

Quality Level

200

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CASP3(836)

General description

The Caspase 3 Fluorometric Assay Kit is based on the hydrolysis of acetyl Asp-Glu-Val-Asp 7-amido-4-methylcoumarin (Ac-DEVD-AMC) by caspase 3, resulting in the release of the fluorescent 7-amino-4-methylcoumarin (AMC). The excitation and emission wavelengths of AMC are 360 nm and 460 nm respectively. The concentration of the AMC released can be calculated from a calibration curve prepared with AMC standards (AMC standard included with the kit).

Application

The Caspase 3 Fluorimetric Assay Kit is designed for the detection and measurement of caspase 3 activity in cell extracts or purified preparations of caspase 3.

Unit Definition

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD per min at pH 7.4 at 25 °C.

Kit Components Only

Product No.
Description
  • Water (17-18 megaOhm) 125 mL

Kit Components Also Available Separately

Product No.
Description
SDS
  • A1086Ac-DEVD-AMC substrate 2.5 mgSDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS
  • A98917-Amino-4-methylcoumarin standard 1 mgSDS

related product

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

N M Martins et al.
Journal of applied toxicology : JAT, 28(3), 337-344 (2007-07-03)
Cisplatin is a potent and widely used chemotherapeutic agent. Nephrotoxicity induced by this drug has been well documented. However, very little information is available on cisplatin-induced hepatotoxicity and its underlying mechanism remains unclear. High doses of cisplatin have been known
Irshad Ali et al.
The Annals of thoracic surgery, 93(1), 282-288 (2011-11-26)
We previously reported that the cytochrome P450 product 20-hydroxyeicosatetraenoic acid has prosurvival effects in pulmonary artery endothelial cells and ex vivo pulmonary arteries. We tested the potential of a 20-hydroxyeicosatetraenoic acid analog N-[20-hydroxyeicosa-5(Z),14(Z)-dienoyl]glycine (20-5,14-HEDGE) to protect against lung ischemic reperfusion
Alfia Khaibullina et al.
Blood cells, molecules & diseases, 86, 102493-102493 (2020-09-15)
Strokes are feared complications of sickle cell disease (SCD) and yield significant neurologic and neurocognitive deficits. However, even without detectable strokes, SCD patients have significant neurocognitive deficits in domains of learning and memory, processing speed and executive function. In these
V A Smith et al.
The British journal of ophthalmology, 88(5), 619-625 (2004-04-20)
Doxycycline is a broad spectrum antibiotic that chelates metal ions and is frequently used as part of the treatment of ocular surface diseases. Its therapeutic value has been ascribed to an ability to inhibit matrix metalloproteinase (MMP) activity and both
Chun-Te Chen et al.
American journal of cancer research, 5(12), 3560-3569 (2016-02-18)
Drug repurposing is a therapeutic strategy that applies drugs to treat different diseases based on new therapeutic function. Carglumic acid (Carbaglu; Orphan Europe) is an orphan drug approved by the FDA for hyperammonemia. Administration of carglumic acid for treatment of
View All Related Papers

Articles

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Apoptosis Assays

Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service