Skip to Content
Merck
All Photos(2)

Documents

C3041

Sigma-Aldrich

Carbonate-Bicarbonate Buffer

capsule

Synonym(s):

Carbonate Buffer Solution

Sign Into View Organizational & Contract Pricing
All Photos(2)

About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

form

capsule

pH

9.3-9.9

solubility

water, high purity: 1 capsule/100 mL

application(s)

diagnostic assay manufacturing

General description

Bicarbonate buffer mostly have a pH close to 7.4. It is the major small intestine buffer.

Application

Carbonate-Bicarbonate Buffer has been used for suspending the lyophilized human plasma sample for microtiter plate biofilm assay and flow cell biofilm assay. It has also been used as a coating buffer for microtiter plates prior to enzyme-linked immunosorbent assay(ELISA).
Prepared buffer is suitable for use in protein coating procedures on multiwell plates or plastic tubes.

Reconstitution

Empty the contents of one capsule in 100 mL of deionized water and dissolve. The contents of one capsule yields 100 ml of 0.05 M carbonate-bicarbonate buffer, pH 9.6 at 25 °C.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

recommended

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H319

Hazard Classifications

Eye Irrit. 2

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Ashfaq Ghumra et al.
PloS one, 6(1), e16414-e16414 (2011-02-10)
Rosetting is a Plasmodium falciparum virulence factor implicated in the pathogenesis of life-threatening malaria. Rosetting occurs when parasite-derived P. falciparum Erythrocyte Membrane Protein One (PfEMP1) on the surface of infected erythrocytes binds to human receptors on uninfected erythrocytes. PfEMP1 is
Mike Burrows et al.
BMC complementary and alternative medicine, 9, 49-49 (2009-12-02)
For almost 2000 years it has been recognized that aqueous extracts from pine cones possess medicinal properties beneficial for the treatment of a broad variety of diseases and conditions. In this report, the ability of an orally administered poly phenylpropanoid-polysaccharide
L von Gersdorff Jørgensen et al.
Journal of fish diseases, 40(12), 1815-1821 (2017-05-27)
The protective effect in rainbow trout (Oncorhynchus mykiss) of an experimental subunit vaccine targeting antigens in the parasite Ichthyophthirius multifiliis has been evaluated and compared to effects elicited by a classical parasite homogenate vaccine. Three recombinant parasite proteins (two produced
Experimental evidence for direct in situ binding of IgM and IgT to early trophonts of Ichthyophthirius multifiliis (Fouquet) in the gills of rainbow trout, Oncorhynchus mykiss (Walbaum)
von Gersdorff J, et al.
Journal of Fish Diseases, 34(10), 749-755 (2011)
T J Caperna et al.
Domestic animal endocrinology, 60, 75-82 (2017-05-30)
A simple, reproducible sandwich, ELISA was developed to measure porcine alpha-1 acid glycoprotein (pAGP, ORM-1) in pig plasma. Porcine AGP isolated from serum was purchased and a polyclonal antisera was prepared in rabbits using the whole pAGP molecule as immunogen.
View All Related Papers

Protocols

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

ELISA Procedures

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service