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C0954

Sigma-Aldrich

Chondroitinase C from Flavobacterium heparinum

lyophilized powder, ≥200 units/mg solid

Synonym(s):

Chondroitin C lyase

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About This Item

CAS Number:
60184-91-0
MDL number:
MFCD00212904
UNSPSC Code:
12352204
NACRES:
NA.54

conjugate

(Glucosaminoglycan)

Quality Level

200

form

lyophilized powder

specific activity

≥200 units/mg solid

storage temp.

−20°C

Related Categories

General description

Enzyme Commission (EC) Number 4.2.2.x

Biochem/physiol Actions

Chondroitinase C acts upon chondroitin sulfate C, releasing unsaturated 6-sulfated disaccharides. The enzyme also acts on hyaluronic acid, producing unsaturated nonsulfated disaccharides. The enzyme is strongly inhibited by NaCl, Fe2+, Co2+ and phosphate ions.

Unit Definition

One unit will form 0.1 μmole of unsaturated uronic acid per hr at pH 8.0 at 25 °C using chondroitin sulfate C as substrate.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Y M Michelacci et al.
The Journal of biological chemistry, 251(4), 1154-1158 (1976-02-25)
A chondroitinase that acts upon chondroitin sulfate C and hyaluronic acid was isolated from Flavobacterium heparinum. This enzyme was seperated from constitutional chondroitinase AC and an induced chondroitinase B also present in extracts of F. heparinum previously grown in the
N Ototani et al.
Carbohydrate research, 70(2), 295-306 (1979-05-01)
Chondroitinase B and chondroitinase C were separated from an extract of Flavobacterium heparinum induced with chondroitin 6-sulfate by using column chromatography on hydroxylapatite. Chondroitinase C was eluted together with the activities of hyaluronidase, delta4,5glycosiduronase, and sulfatase. The latter two activities

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