The A4540 Anti-Mouse IgM (μ-chain specific)−Agarose antibody, produced in goat IgG fraction of antiserum, is supplied as a saline suspension and not a phosphate buffer. For the equilibration step, a standard 0.01M Phosphate buffer at pH 7.2 can be used.
A4540
Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat
IgG fraction of antiserum, saline suspension
Synonym(s):
Anti-Mouse IgM, Goat Anti-Mouse IgM
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About This Item
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biological source
goat
Quality Level
conjugate
agarose conjugate
antibody form
IgG fraction of antiserum
antibody product type
secondary antibodies
clone
polyclonal
form
saline suspension
species reactivity
mouse
extent of labeling
≥5 mg per mL
technique(s)
Ouchterlony double diffusion: suitable
capacity
0.4 mg binding capacity
storage temp.
2-8°C
target post-translational modification
unmodified
General description
IgM exists as a pentamer and is secreted by peritoneal B cells. IgM is a highly conserved antibody in vertebrates and is expressed early during immune response.
IgM is a glycoprotein antibody that regulates humoral immune responses. Mouse IgM is involved in modulating B cell memory. This antibody isotype has also been implicated in the development of autoimmune responses associated with the pathogenesis of type 1 diabetes in mice.
Goat anti-mouse IgM (μ-chain specific)-agarose antibody is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM myeloma proteins.
Goat anti-mouse IgM (μ-chain specific)-agarose antibody is specific for mouse IgM when tested against purified mouse IgA, IgG (all subclasses), and IgM myeloma proteins.
Application
Anti-Mouse IgM (μ-chain specific)−Agarose antibody produced in goat has been used in agarose binding assay in primary splenic cells and in immunoprecipitation of soluble lens protein and myelogenous leukemia cell lines.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Chromatin immunoprecipitation (1 paper)
Chromatin immunoprecipitation (1 paper)
Goat anti-mouse IgM (μ-chain specific)-agarose antibody has been used for immunoprecipitation and ChIP assays. The product can also be used for Ouchterlony double diffusion applications.
To isolate chimeric proteins that were fused with the IgM heavy chain, CHO supernatant was ran over a column packed with agarose-linked goat anti-mouse IgM mu chain specific.
Biochem/physiol Actions
IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules. IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM linked agarose resin have been tested for efficient conjugate binding.
Physical form
Suspension in 0.5 M NaCl containing preservative.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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In what type of buffer is the sodium phosphate buffer antibody supplied, and what buffer should be used?
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