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90101

Sigma-Aldrich

Ampliflu™ Red

for fluorescence, ≥98.0% (HPLC)

Synonym(s):

10-Acetyl-3,7-dihydroxyphenoxazine

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About This Item

Empirical Formula (Hill Notation):
C14H11NO4
CAS Number:
119171-73-2
Molecular Weight:
257.24
MDL number:
MFCD00467861
UNSPSC Code:
12352108
PubChem Substance ID:
329769846
NACRES:
NA.32

grade

for fluorescence

Assay

≥98.0% (HPLC)

form

powder

fluorescence

λex 571 nm; λem 585 nm in DMSO

storage temp.

2-8°C

SMILES string

CC(=O)N1c2ccc(O)cc2Oc3cc(O)ccc13

InChI

1S/C14H11NO4/c1-8(16)15-11-4-2-9(17)6-13(11)19-14-7-10(18)3-5-12(14)15/h2-7,17-18H,1H3

InChI key

PKYCWFICOKSIHZ-UHFFFAOYSA-N

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Application

Ampliflu™ Red has been used to study mitochondrial abnormalities and oxidative stress related to Parkinson’s Disease. Ampliflu™ Red assay was suitable as an alternative indicator assay for detecting the inhibition of hTPO (human thyroid peroxidase) activity. Phospholipase D, an essential cellular process ingredient, used Ampliflu™ Red (10-Acetyl-3,7-dihydroxyphenoxazine) to determine its activity.Ampliflu™ Red is used for the following applications:
  • Quantification of inflammatory mediators
  • Determination of free and esterified cholesterol contents
  • Evaluation of compounds as substrates for GABA-AT compounds

Legal Information

Ampliflu is a trademark of Sigma-Aldrich Co. LLC

related product

Product No.
Description
Pricing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302

Precautionary Statements

P264 - P270 - P301 + P312 - P501

Hazard Classifications

Acute Tox. 4 Oral

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Kazuo Sugiyama et al.
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Scientific reports, 9(1), 11447-11447 (2019-08-09)
Macrophages may be a crucial aspect of diabetic complications associated with the inflammatory response. In this study, we examined how hyperglycaemia, a common aspect of diabetes, modulates bone marrow-derived macrophages (BMDMs) under an inflammatory stimulus. To perform this study, BMDMs
Yu Ji et al.
Chemistry (Weinheim an der Bergstrasse, Germany), 24(63), 16865-16872 (2018-08-29)
Cetyl-trimethylammonium bromide (CTAB) is a widely used cationic surfactant that is biodegradable in nature. CTAB biodegradation requires hydroxylation in the first step, which is rate-limiting and crucial for solubility in water. In this study, the OmniChange multi-site mutagenesis method was
Barae Jomaa et al.
ALTEX, 32(3), 191-200 (2015-03-31)
A simple and rapid luminometric assay for the detection of chemical inhibitors of human thyroid peroxidase (hTPO) activity was developed and validated with 10 model compounds. hTPO was derived from the human thyroid follicular cell line Nthy-ori 3-1 and its
Maria Luisa Di Paolo et al.
Journal of enzyme inhibition and medicinal chemistry, 34(1), 740-752 (2019-03-05)
Fourteen polyamine analogues, asymmetric or symmetric substituted spermine (1-9) or methoctramine (10-14) analogues, were evaluated as potential inhibitors or substrates of two enzymes of the polyamine catabolic pathway, spermine oxidase (SMOX) and acetylpolyamine oxidase (PAOX). Compound 2 turned out to
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