Skip to Content
Merck
All Photos(1)

Documents

SBR00025

Sigma-Aldrich

Novobiocin Ready Made Solution

100 mg/mL in water

Synonym(s):

Novobiocin Ready Made Solution

Sign Into View Organizational & Contract Pricing


About This Item

Linear Formula:
C31H35N2NaO11
Molecular Weight:
634.61
UNSPSC Code:
51286504
NACRES:
NA.76

biological source

microbial

Assay

≥90%

form

liquid

concentration

100 mg/mL in water

color

faint yellow to dark brown

antibiotic activity spectrum

Gram-negative bacteria
Gram-positive bacteria
mycobacteria
neoplastics

Mode of action

DNA synthesis | interferes
enzyme | inhibits

storage temp.

−20°C

General description

Novobiocin, also known as albamycin or cathomycin, is an aminocoumarin antibiotic commonly derived from Streptomyces Sp. It exerts its antibacterial activity by inhibiting Topo II α, which corresponds to bacterial DNA gyrase. It is effective against both gram-positive and gram-negative bacteria and is significant in research involving highly beta-lactam-resistant pneumococcal infections and anti-orthopoxvirus applications. Functioning as a competitive inhibitor in the ATPase reaction catalyzed by GyrB, Novobiocin is employed in microbiology and cell biology research to effectively prevent the growth of bacterial contaminants.

Application

Novobiocin has been used:
  • in cell culture systems to treat mycoplasma contaminations.[3]
  • in a research to where it has exhibited anti-cancer effects by inhibition of Hsp90 (heat shock protein)[4],[5]

Biochem/physiol Actions

Mode of Action: Inhibits DNA synthesis by inhibiting the enzyme Topoisomerase II

Activity spectrum: Active against gram-positive and gram-negative bacteria

Features and Benefits

  • Ready available solution reduce the need for preparation time
  • Commonly used in Cell Biology and Biochemical applications
  • High quality antibiotic suitable for mulitple research applications

Preparation Note

Novobiocin Ready Made Solution is light sensitive. Thaw this product at room temperature. It is recommended to avoid repeated freeze thaw cycles of this product. The suggested concentration of Novobiocin for cell culture assays is 50-1000 μg/mL,,, therefore, it is recommended to dilute the Ready Made Solution 1:100-2000.

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Hazard Classifications

Skin Sens. 1

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Mycoplasmas in tissue culture.
Macpherson, I.
Journal of Cell Science, 1, 145-168 (1966)
Effects of novobiocin, coumermycin A1, clorobiocin, and their analogs on Escherichia coli DNA gyrase and bacterial growth.
Hooper, D. C., Wolfson, J. S., McHugh, G. L., Winters, M. B., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 22, 662-671 (1982)
Novobiocin decreases SMYD3 expression and inhibits the migration of MDA-MB-231 human breast cancer cells.
Luo, X. G., Zou, J. N., Wang, S. Z., Zhang, T. C., & Xi, T.
IUBMB Life, 62, 194-199 null
Novobiocin antagonism of amastigotes of Trypanosoma cruzi growing in cell-free medium.
Pate, P. G., Wolfson, J. S., McHugh, G. L., Pan, S. C., & Swartz, M. N.
Antimicrobial Agents and Chemotherapy, 29, 426-431 (1986)
Synthesis and biological evaluation of arylated novobiocin analogs as Hsp90 inhibitors.
Kusuma, B. R., Duerfeldt, A. S., & Blagg, B. S.
Bioorganic & Medicinal Chemistry Letters, 21, 7170-7174 (2011)

Related Content

Discover cancer research resources with modeling and profiling tools for cell culture, genomics, biomarkers, and more to help maximize your cancer research.

Discover cancer research resources with modeling and profiling tools for cell culture, genomics, biomarkers, and more to help maximize your cancer research.

Discover cancer research resources with modeling and profiling tools for cell culture, genomics, biomarkers, and more to help maximize your cancer research.

Discover cancer research resources with modeling and profiling tools for cell culture, genomics, biomarkers, and more to help maximize your cancer research.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service