11815016001
Roche
Anti-HA Affinity Matrix
from rat IgG1
Synonym(s):
affinity matrix, anti-ha
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About This Item
UNSPSC Code:
12352203
Recommended Products
biological source
rat
Quality Level
conjugate
agarose conjugate
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
3F10, monoclonal
form
slurry
packaging
pkg of 1 mL (settled resin volume)
manufacturer/tradename
Roche
isotype
IgG1
epitope sequence
YPYDVPDYA
capacity
2-8 nmol/mL binding capacity
storage temp.
2-8°C
General description
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal)
Specificity
Anti-HA High Affinity antibody (clone 3F10) recognizes the 9-amino acid sequence YPYDVPDYA, derived from the human influenza hemagglutinin (HA) protein. This epitope is also recognized in fusion proteins that are derived from mammalian, bacterial, and yeast expression vectors, regardless of its position (N-terminal, C-terminal or internal).
Immunogen
Amino acids 98-106 from the human influenza virus hemagglutinin protein
Application
Following immunoprecipitation or purification, the tagged protein of interest may be analyzed by:
- Western blotting using the Anti-HA antibody
- Silver staining (or similar protein stain)
- In coimmunoprecipitation.
Use Anti-HA Affinity Matrix for:
- Immunoprecipitation of HA-tagged proteins from mammalian, bacterial, and yeast cell extracts
- Affinity column purification of HA-tagged proteins from crude protein extracts
Features and Benefits
- Achieve enhanced specificity and reduced cross-reactivity of Anti-HA High Affinity.
- Use nondenaturing conditions for elution of your HA-tagged protein of choice.
- Purify even rarely expressed HA-tagged proteins.
- Highly specific to YPYDVPDYA.
- Suitable for purification of proteins containing HA-epitop as N-terminal, C-terminal or internal fusion.
- Applicable with crued cell extracts from mammalian, bacterial and yeast expression systems.
Quality
Each lot of Anti-HA Affinity Matrix is tested for its ability to purify a HA-tagged protein expressed in stably transfected cells from a crude mammalian extract. The immunoprecipitate is analyzed by western blot using Anti-HA, High Affinity antibody.
Physical form
1 ml settled resin of Anti-HA Affinity Matrix in PBS containing 0.09% sodium azide (w/v); 2 ml suspension equals to 1 ml bed volume. One plastic column with top and bottom caps is included.
Analysis Note
Yield of 9 nmol purified protein/ml affinity matrix was determined using a whole-cell bacterial extract containing expressed HA-tagged bacterial alkaline phosphatase.
Other Notes
For life science research only. Not for use in diagnostic procedures.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
does not flash
Flash Point(C)
does not flash
Certificates of Analysis (COA)
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Protocols
To concentrate the protein eluate, Roche recommends following the immunoprecipitation protocol given in the package insert from step 2 to 5. Use 50 μl of resuspended Anti-HA Affinity Matrix in a microcentrifuge tube.
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