Please refer to the "Terrific Broth" substance page for alternatives:
https://www.sigmaaldrich.com/substance/terrificbroth1234598765
T5574
Synonym(s):
TB medium, Tartoff – Hobbs medium
for molecular biology
Liquid Medium
sterile; 0.2 μm filtered
liquid
microbiological culture: suitable
microbiology
room temp
nonselective for Escherichia coli
nonselective for coliforms
12 - Non Combustible Liquids
WGK 2
Not applicable
Not applicable
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Preparation of Plasmid DNA by Alkaline Lysis with SDS: Maxipreparation between Cold Spring Harbor Laboratory Press and our research team.
General protocols for growth of competent cells in microbial medium.
What are the alternatives for T5574?
Please refer to the "Terrific Broth" substance page for alternatives:
https://www.sigmaaldrich.com/substance/terrificbroth1234598765
Helpful?
Which bacterial culture medium is the best choice for my application?
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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