SRP8001

Calreticulin human

recombinant, expressed in E. coli, His tagged, >90% (SDS-PAGE)

• Synonym(s): CRP55, Calregulin, ERp60, Endoplasmic reticulum resident protein 60, grp60
• UNSPSC Code: 12352200
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• tag: His tagged
• Assay: >90% (SDS-PAGE)
• form: liquid
• mol wt: ~55 kDa by SDS-PAGE
• packaging: pkg of 10 μg
• storage condition: avoid repeated freeze/thaw cycles
• technique(s): calcium flux assay: suitable
• impurities: <1 EU/μg endotoxin, tested
• UniProt accession no.: P27797
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... CALR(811)

Description

General description

Calreticulin (CALR) is expressed in the endoplasmic reticulum. The gene encoding it is localized on human chromosome 19p13.13.

Application

Human calreticulin has been used in a study to determine the effects of uncoupling protein 4 (UCP4) on the expression of proteins in the store-operated Ca²⁺ entry pathway.

Biochem/physiol Actions

Calreticulin is involved in regulation of intracellular Ca²⁺ homoeostasis and ER Ca²⁺ capacity. It constitutes together with calnexin and ERp57 the ′calreticulin/calnexin cycle′ that is responsible for folding and quality control of newly synthesized glycoproteins. Calreticulin has been implicated to play a role in many biological systems, including functions inside and outside the ER, indicating that the protein is a multi-process molecule. Recently, Calreticulin was shown to enhance the merger of macrophages and tumor cells, increasing phagocytosis.

Physical form

Solution in 55 mM TRIS-HCl, pH 8.2, containing 150 mM NaCl.

Other Notes

Human calreticulin (aa18-417) is fused at the C-terminus to a His-tag.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable