SRP4134

IL-3 human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

• Synonym(s): HCGF, IL-3, Interleukin-3, MCGF (Mast cell growth factor), MGC79398, Multi-CSF, P-cell stimulation factor
• MDL number: MFCD00147276
• UNSPSC Code: 12352202
• NACRES: NA.32

Properties

• biological source: human
• recombinant: expressed in E. coli
• Assay: ≥98% (HPLC); ≥98% (SDS-PAGE)
• form: lyophilized
• mol wt: ~15.0 kDa
• packaging: pkg of 10 μg
• impurities: endotoxin, tested
• NCBI accession no.: P08700
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... IL3(3562)

Description

General description

IL-3 is a species-specific colony-stimulating factor that stimulates colony formation of megakaryocytes, neutrophils, and macrophages from bone marrow cultures. Recombinant human IL-3 is a 15.0 kDa protein containing 133 amino acid residues.

Biochem/physiol Actions

Interleukin-3 (IL-3) is a hematopoietic growth factor. The gene encoding it is localized on human chromosome 5. It has a role in cell proliferation and activates different signaling cascades. It is crucial for the differentiation of hematopoietic-derived endothelial progenitor cells (EPCs). The differentiation of erythroid cells, mast cells and granulocytes is stimulated by IL-3. The protein has been linked to schizophrenia.

Physical form

Lyophilized without additives.

Reconstitution

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles.

Reconstitute in water to a concentration of 0.1-1.0 mg/mL. The solution can then be diluted into other aqueous buffers.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable