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MAK121

Sigma-Aldrich

Neuraminidase Activity Assay Kit

Neuraminidase Activity Assay Kit
1 of 1 reviewers received a sample product or took part in a promotion

sufficient for 100 colorimetric or fluorometric tests

Synonym(s):

Sialidase Activity Assay Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
Pricing and availability is not currently available.

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

pharmaceutical

detection method

colorimetric
fluorometric

shipped in

dry ice

storage temp.

−20°C

General description

Neuraminidase, also known as Sialidase, is an enzyme that hydrolyzes terminal sialic acid residues on poly-saccharide chains. It is predominantly expressed in microorganisms such as bacteria and viruses. Cleavage of sialic acid residues by neuraminidase is believed to play several roles in infection by influenza viruses. It is thought to assist in the penetration of mucosal linings, the invasion of target cells, the elution of progeny viruses from infected cells, and the prevention of self-aggregation. Thus, neuraminidase is an important target for influenza drug development and simple, direct, and automation-ready procedures for measuring neuraminidase activity find wide applications in research and drug discovery.

Application


  • 5-Nitrobenzo[c][1, 2, 5]selenadiazole as therapeutic agents in the regulation of oxidative stress and inflammation induced by influenza A(H1N1)pdm09 in vitro and in vivo.: This research utilized the Neuraminidase Activity Assay Kit to explore therapeutic agents that regulate oxidative stress and inflammation, showcasing its crucial role in antiviral research and potential pharmacological applications (Guo et al., 2023).

  • Potency of bacterial sialidase Clostridium perfringens as antiviral of Newcastle disease infections using embryonated chicken egg in ovo model.: Highlights the kit′s use to measure and observe sialidase enzyme activity to assess the antiviral potential of bacterial enzymes against poultry diseases, contributing to the development of novel biocontrol methods (Kurnia et al., 2022).

  • Molecular Docking Studies and Biological Evaluation of Berberine-Benzothiazole Derivatives as an Anti-Influenza Agent via Blocking of Neuraminidase.: Utilizes neuraminidase assay kit to evaluate the efficacy of synthesized compound′s neuraminidase activity, offering a path towards the design of new antiviral drugs with enhanced specificity and potency (Kumar et al., 2021).

Features and Benefits

Compatible with high-throughput handling systems.

Suitability

Suitable for the detection of neuraminidase activity in biological samples and for the screening of neuraminidase inhibitors.

Principle

In this assay, neuraminidase activity is determined by an enzymatic assay in which sialic acid released by neuraminidase results in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product directly proportional to neuraminidase activity in the sample. The linear detection range at 37 °C is 0.1-10 units/L for colorimetric assays and 0.01-2 units/L for fluorometric assays.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

10 - Combustible liquids

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
121CE10A09
121CE08A12
121CE06A03
121CE03A19
121CE10A09

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Irene Gómez Delgado et al.
Frontiers in immunology, 12, 641656-641656 (2021-03-30)
Haemolytic Uraemic Syndrome associated with Streptococcus pneumoniae infections (SP-HUS) is a clinically well-known entity that generally affects infants, and could have a worse prognosis than HUS associated to E. coli infections. It has been assumed that complement genetic variants associated
Alfred T Harding et al.
mBio, 8(3) (2017-06-08)
Influenza virus vaccine production is currently limited by the ability to grow circulating human strains in chicken eggs or in cell culture. To facilitate cost-effective growth, vaccine strains are serially passaged under production conditions, which frequently results in mutations of
Aurora Salamanca et al.
Antiviral chemistry & chemotherapy, 29, 20402066211063391-20402066211063391 (2021-11-30)
Seasonal flu is caused by influenza infection, a virus that spreads easily in human population with periodical epidemic outbreaks. The high mutational rate of influenza viruses leads to the emergence of strains resistant to the current treatments. Due to that
Rose T Byrne-Nash et al.
NPJ vaccines, 4, 3-3 (2019-01-25)
Neuraminidase (NA) immunity leads to decreased viral shedding and reduced severity of influenza disease; however, NA content in influenza vaccines is currently not regulated, resulting in inconsistent quality and quantity of NA that can vary from manufacturer to manufacturer, from
Ryan Septa Kurnia et al.
Journal of advanced veterinary and animal research, 9(2), 335-345 (2022-07-28)
The Newcastle disease virus (NDV) is an infectious disease that causes very high economic losses due to decreased livestock production and poultry deaths. The vaccine's ineffectiveness due to mutation of the genetic structure of the virus impacts obstacles in controlling
View All Related Papers
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Questions

1–3 of 3 Questions  

  1. Is it possible to use the Neuraminidase Activity Assay Kit (MAK121-1KT) for testing bacterial cultures, given that references indicate the use of plasma, serum, and purified enzymes as samples?

    1 answer

    1. The kit has been verified for use with bacterial cultures. Although there isn't a tailored sample preparation protocol for bacterial cultures, the following general cell lysate preparation method is suggested:
      To prepare cell samples, first, suspend approximately two million harvested cells (2 × 10^6) in 400 μL of PBS on ice. Cell lysis can be accomplished by either using a Dounce homogenizer for 10-20 passes while on ice or through sonication in an ice-water bath. The effectiveness of lysis should be verified under a microscope. After lysis, centrifuge the homogenate at 14,000 g for 10 minutes and then transfer the clear supernatant to a clean tube. It is recommended to run a pilot assay with different sample dilutions to identify a dilution that falls within the linear range of the standard curve for accurate assays. If immediate analysis is not possible, most samples can be preserved at -80°C.

      Helpful?

  2. What type of sialic acid linkage is present in this kit? Specifically, is it an α2,3- or α2,6-linkage?

    1 answer

    1. The substrate utilized is fetuin, which is a heavily sialylated protein derived from fetal bovine serum. The specific type of sialic acid linkage present in this substrate has not been characterized. However, fetuin typically exhibits two types of linkages: α2,3- and α2,6-linkages. It is more common for α2,3-linkages to be present in fetuin substrates, which suggests that experiments targeting this linkage type are likely to be successful. Nonetheless, it is not possible to provide a definitive statement on the exact type of linkage present.

      Helpful?

  3. Hallo, welches MQ Level hat das Material MAK121?

    1 answer

    1. These kits is considered an out of scope in terms of the MQ Classification system, but are handled and supported similarly to the MQ100 level products.

      Helpful?

Reviews

1 of 1 reviewers received a sample product or took part in a promotion

Active Filters

  1. Ire
    • Reviews 2
    • Votes 0
    3 out of 5 stars.

    .

    Good kit but very expensive and very few samples can be run with one kit.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for taking the time to leave a review! We are sorry to hear that your experience did not meet expectations. We would like to learn more about your experience with this kit. When you have a moment, please contact us by visiting https://www.sigmaaldrich.com/support/customer-support and submit a "Report Product Issue" ticket. We look forward to hearing from you soon!

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