M3795

IgM, Kappa from murine myeloma

clone TEPC 183, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Mouse IgM-κ
• MDL number: MFCD00283897
• UNSPSC Code: 12352203
• NACRES: NA.46

Properties

• biological source: mouse
• Quality Level: 300
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• clone: TEPC 183, monoclonal
• Assay: ≥90% (HPLC)
• form: buffered aqueous solution
• concentration: 2.0-2.2 mg/mL
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Determined by the absorbance at 280 nm (E^1%/₂₈₀ = 11.8). Each vial contains at least 1 mg of purified myeloma protein.

IgM antibodies are present as pentamers in the serum and are produced in response to antigens[1] IgM, κ from murine myeloma is produced by TEPC 183 tumor line introduced subcutaneously in BALB/c mice.

IgM is a highly conserved antibody in vertebrates and is expressed early during immune response.[2] It is secreted by peritoneal B cells.[3]

Specificity

The TEPC 183 tumor line is pristine-induced plasmacytoma-originated and carried subcutaneously in BALB/c mice. The hapten binding specificity of the TEPC 183 line has not been determined. It specifically recognizes mouse IgM. Identity and purity of the immunoglobulin is established by immunoelectrophoresis.

Application

IgM, Kappa from murine myeloma has been used in:

• sandwich enzyme linked immunosorbent assay (ELISA)[4][5]
• flow cytometry[6]
• dot-immunobinding assay[7]

Biochem/physiol Actions

IgM plays a key role in the engulfing of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections.[3] IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.[8] IgM has sites for N-linked glycosylation, with sugars namely, mannose, galactose, N-acetyl glucosamine and sialic acid.[9] IgM linked agarose resins have been tested for efficient conjugate binding.[10]

Physical form

Solution in 0.05 M Tris, 0.5 M sodium chloride, pH 8.0, containing 0.02% sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves