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I4510

Sigma-Aldrich

Iminodiacetic acid Sepharose

aqueous ethanol suspension

Synonym(s):

Iminodiacetic acid Agarose, IDA Resin

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About This Item

MDL number:
UNSPSC Code:
23151817
PubChem Substance ID:
NACRES:
NA.56

form

aqueous ethanol suspension

matrix

Sepharose 6B Fast Flow

matrix activation

epoxy

matrix attachment

amino

matrix spacer

7 atoms

capacity

22-30 μmol/mL binding capacity (Zn2+)

storage temp.

2-8°C

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General description

I4510-50ML′s updated product number is GE17-0575-01
Iminodiacetic acid Sepharose is a chelating resin used for the purification of proteins.

Application

Iminodiacetic acid Sepharose has been slurry-packed into a tared polypropylene column for immobilized metal-ion affinity chromatography (IMAC) and for the preparation of the chelating sepharose column. It is an important component of metal affinity chromatography which immobilizes transition metal ions.
Iminodiacetic acid Sepharose is used is affinity chromatography, protein chromatography and chelating resins. Iminodiacetic acid Sepharose has been used to report a new procedure for the purification of two human monoclonal anti-HIV (human immunodeficiency virus) antibodies (mAbs 2G12 and 4E10).

Physical form

Suspension in 20% ethanol

Legal Information

Sepharose is a trademark of Cytiva

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Description
Pricing

replaced by

Pictograms

Flame

Signal Word

Warning

Hazard Statements

Hazard Classifications

Flam. Liq. 3

Storage Class Code

3 - Flammable liquids

WGK

WGK 1

Flash Point(F)

96.8 °F

Flash Point(C)

36 °C


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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B Sutter et al.
Protein expression and purification, 19(1), 53-56 (2000-06-02)
The superoxide dismutases (EC 1.15.1.1) are a family of enzymes that catalyze the dismutation of superoxide radical anion to dioxygen and hydrogen peroxide. The active site contains a critical metal ion such as manganese, iron, or copper. The copper-containing protein
A Naeem et al.
Biochemistry. Biokhimiia, 71(1), 56-59 (2006-02-07)
Concanavalin A and a mannose-specific lectin could be precipitated specifically from extracts of jack bean and Cajanus cajan seeds, respectively, using metal charged EGTA. Single step purification of the lectins was also possible using iminodiacetic acid-Sepharose charged with metal ions.
Immobilized iminodiacetic acid metal peptide complexes. Identification of chelating peptide purification handles for recombinant proteins
Smith MC, et al.
Inorganic Chemistry, 26(12), 1965-1969 (1987)
Identification and quantification of histidine-rich glycoprotein (HRG) in the blood plasma of six marine bivalves.
Abebe, A.T., et al.
Comp. Biochem. Physiol., B: Comp. Biochem., 147(1), 74-81 (2007)
Pierluigi Pilla et al.
Biosensors & bioelectronics, 31(1), 486-491 (2011-12-06)
We present a fast and effective method for anchoring bioreceptors to optical waveguides exhibiting a poorly reactive polymer interface and that have to be minimally perturbed with respect to their design. The study originated from the need to biofunctionalize a

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