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CS0780

Sigma-Aldrich

β-N-Acetylglucosaminidase Assay Kit

sufficient for 50 reactions (1 mL), sufficient for 500 reactions (100 μL)

Synonym(s):

Beta-NAG Activity Assay Kit

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About This Item

EC Number:
222-398-7
UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 50 reactions (1 mL)
 sufficient for 500 reactions (100 μL)

Quality Level

200

shipped in

wet ice

storage temp.

2-8°C

Gene Information

human ... NAGLU(4669)

Application

The kit provides the reagents required for a fast and convenient detection of β-N-Acetylglucosaminidase activity in cell lysates, tissue homogenates or purified enzyme. It is a useful tool for the detection of lysosomes in fractionated cell/tissue samples.

Biochem/physiol Actions

N-acetyl-D-glucosaminidase (NAG) in mammals is a lysosomal enzyme, which takes part in the intracellular degradation of glycolipids and glycoproteins. High activities of this enzyme have been detected in human kidney, lung and liver lysosomes. Elevated levels of serum NAG are associated with certain forms of cancer.

Analysis Note

The kit assay is based on the hydrolysis of NAG substrate (NP-GLcNAc) by the enzyme. The enzymatic hydrolysis of the substrate liberates p-nitrophenylate ion. The reaction product is detected colorimetrically at 405 nm.

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Kit Components Only

Product No.
Description
  • Dilution Buffer 8 mL
  • 4-Nitrophenyl-N-acetyl-β-D-glucosaminide 50 mg
  • Citrate Buffer Solution, 0.09 M 100 mL
  • p-Nitrophenol Standard Solution, 10 mM 1 mL
  • β-N-Acetylglucosaminidase from Jack beans 1 vial
  • Sodium carbonate 5 g

related product

Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H319,H351,H373

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - STOT RE 2 Oral

Target Organs

Liver,Kidney

Storage Class Code

11 - Combustible Solids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Miguel A Lanaspa et al.
Journal of the American Society of Nephrology : JASN, 25(11), 2526-2538 (2014-05-31)
Diabetes is associated with activation of the polyol pathway, in which glucose is converted to sorbitol by aldose reductase. Previous studies focused on the role of sorbitol in mediating diabetic complications. However, in the proximal tubule, sorbitol can be converted
Shanshan Wang et al.
Biochemical and biophysical research communications, 483(1), 45-51 (2017-01-09)
Activation of endothelial cells plays a key role in septic acute kidney injury (AKI). This study investigated the role of miRNA in endothelial-induced tubular cell injury in sepsis. Circulating endothelial cells (CECs) from septic AKI, non-septic AKI, septic non-AKI patients
Na Zhang et al.
Cell death & disease, 9(2), 230-230 (2018-02-16)
Glycogen synthase kinase-3β (GSK-3β) is a ubiquitously expressed serine/threonine kinase involved in a variety of functions ranging from the control of glycogen metabolism to transcriptional regulation. We recently demonstrated that GSK-3β inhibition triggered ASK1-JNK-dependent apoptosis in human hepatocellular carcinoma (HCC)
Qiuyuan Yin et al.
The Journal of cell biology, 219(8) (2020-07-15)
Lysosomes are degradation and signaling organelles that adapt their biogenesis to meet many different cellular demands; however, it is unknown how lysosomes change their numbers for cell division. Here, we report that the cyclin-dependent kinases CDK4/6 regulate lysosome biogenesis during
Xiaoting Chen et al.
Journal of cellular and molecular medicine, 23(6), 4290-4300 (2019-04-12)
Impaired autophagic degradation of intracellular lipids is causally linked to the development of non-alcoholic steatohepatitis (NASH). Pharmacological agents that can restore hepatic autophagic flux could therefore have therapeutic potentials for this increasingly prevalent disease. Herein, we investigated the effects of
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Centrifugation separates organelles based on size, shape, and density, facilitating subcellular fractionation across various samples.

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