Dulbecco′s Phosphate Buffered Saline (DPBS) is intended for use in the maintenance of mammalian cells where a chemically defined balanced salt solution provides an environment that will maintain the structural and physiological integrity of cells in vitro. DPBS is commonly used in cell enumerations as a diluent, for rinsing cells and as a buffer in many chromatographic procedures. DPBS also is used to wash and resuspend cells during the dissociation process, where the presence of calcium and magnesium can inhibit trypsin activity. For this application, use DPBS Modified, which is formulated without calcium and magnesium salts.
The short-chain fatty acid propionate is a potent inhibitor of molds that is widely used as a food preservative and endogenously produced by gut microbiota. Although generally recognized as safe by the U.S. Food and Drug Administration, the metabolic effects
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