49752

2-Phospho-L-ascorbic acid trisodium salt

≥95.0% (HPLC)

• Synonym(s): L-Ascorbic acid 2-phosphate trisodium salt, Sodium L-ascorbyl-2-phosphate
• Empirical Formula (Hill Notation): C₆H₆Na₃O₉P · xH₂O
• CAS Number: 66170-10-3
• Molecular Weight: 322.05 (anhydrous basis)
• MDL number: MFCD03095479
• UNSPSC Code: 12352205
• NACRES: NA.79

Properties

• biological source: synthetic
• Quality Level: 200
• Assay: ≥95.0% (HPLC)
• form: powder
• technique(s): cell culture | mammalian: suitable
• impurities: ≤7.5% water (Karl Fischer)
• color: white to off-white
• pH: 9.0-9.5 (25 °C, 30 g/L in H₂O)
• mp: 260 °C
• anion traces: chloride (Cl^-): ≤50 mg/kg; sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤10 mg/kg; As: ≤2 mg/kg; Ba: ≤5 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤10 mg/kg; Mg: ≤10 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• SMILES string: [Na+].[Na+].[Na+].[P](=O)([O-])([O-])OC1=C([C@H](OC1=O)[C@@H](O)CO)[O-]
• InChI: 1S/C6H9O9P.3Na/c7-1-2(8)4-3(9)5(6(10)14-4)15-16(11,12)13;;;/h2,4,7-9H,1H2,(H2,11,12,13);;;/q;3*+1/p-3/t2-,4+;;;/m0.../s1
• InChI key: YRWWOAFMPXPHEJ-OFBPEYICSA-K

Description

Application

2-Phospho-L-ascorbic acid trisodium salt has been used as medium supplement in Dulbecco′s Modified Eagle Medium (DMEM) for adipose-derived stem cells, human mesenchymal stem cells and osteoblast cells.

Biochem/physiol Actions

L-Ascorbic acid 2-phosphate (AA2P) is used in biocatalytic dephosphorylation for electric power generation and electrochemical detection assays. L-Ascorbic acid 2-phosphate may be used in cell differentiation and tissue engineering applications. Asc-2P is used in studies of gene repression, such as the repression of dihydrotestosterone-induced dickkopf-1 expression.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves