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Sigma-Aldrich

5-Carboxy-X-rhodamine N-succinimidyl ester

BioReagent, suitable for fluorescence

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About This Item

Empirical Formula (Hill Notation):
C37H33N3O7
CAS Number:
209734-74-7
Molecular Weight:
631.67
MDL number:
MFCD07437880
UNSPSC Code:
12352116
PubChem Substance ID:
329756168
NACRES:
NA.32

product line

BioReagent

form

powder

solubility

DMF: soluble
acetonitrile: soluble

fluorescence

λex 575 nm; λem 600 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence

storage temp.

−20°C

SMILES string

[O-]C(=O)c1cc(ccc1-c2c3cc4CCCN5CCCc(c45)c3[o+]c6c7CCCN8CCCc(cc26)c78)C(=O)ON9C(=O)CCC9=O

InChI

1S/C37H33N3O7/c41-29-11-12-30(42)40(29)47-37(45)22-9-10-23(26(19-22)36(43)44)31-27-17-20-5-1-13-38-15-3-7-24(32(20)38)34(27)46-35-25-8-4-16-39-14-2-6-21(33(25)39)18-28(31)35/h9-10,17-19H,1-8,11-16H2

InChI key

BTTBJYLMDASSAS-UHFFFAOYSA-N

Application

5-Carboxy-X-rhodamine N-succinimidyl ester (ROX-SE) is used as an amine coupling reagent to form 5-carboxy-X-rhodamine (ROX) derivatized compounds such as proteins, nucleic acids and drugs that may be analyzed by fluorescence resonance energy transfer (FRET) and fluorescence quenching applications.Labeling reagent for preparation of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing.

Other Notes

Labeling reagent for prepn. of charge-modified dye-labeled ddNTPs to "direct-load" DNA sequencing

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H335

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Target Organs

Respiratory system

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

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Patrick J Finn et al.
Nucleic acids research, 30(13), 2877-2885 (2002-06-28)
A novel series of charge-modified, dye-labeled 2',3'-dideoxynucleoside-triphosphate terminators were synthesized and evaluated as reagents for DNA sequencing. These terminators possess an advantage over existing reagents in that no purification is required to remove unreacted nucleotide or associated breakdown products prior
Xia Xiang et al.
Analytica chimica acta, 751, 155-160 (2012-10-23)
The development of simple and inexpensive DNA detection strategy is very significant for droplet-based microfluidic system. Here, a droplet-based biosensor for multiplexed DNA analysis is developed with a common imaging device by using fluorescence-based colorimetric method and a graphene nanoprobe.
Ting Qiu et al.
The Analyst, 135(9), 2394-2399 (2010-08-03)
A new strategy for quantitatively detecting micrococcal nuclease (MNase) is proposed using electrostatic interaction-based fluorescence resonance energy transfer (FRET) between positively charged QDs and negatively charged dye-labeled single-stranded DNA (dye-ssDNA). Herein, we have made our attempt to develop a strategy
Yunfeng Xia et al.
The Analyst, 137(24), 5705-5709 (2012-10-27)
We report a sensitive approach for SERS detection of cytochrome c using target binding-induced conformational changes of signal transduction probe (STP). STP labeled with a SERS-active molecule, carboxy-X-rhodamine (ROX), is immobilized on the substrate where the formation of a rigid
Jonathan F Lovell et al.
Journal of the American Chemical Society, 133(46), 18580-18582 (2011-10-27)
Photodynamic therapy (PDT) stands to benefit from improved approaches to real-time treatment monitoring. One method is to use activatable photosensitizers that can both induce cell death (via singlet oxygen) and monitor it (via caspase detection). Here, we report porphyrins as
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