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Supelco

NTA - Atto 488

BioReagent, suitable for fluorescence, ≥80% (sum of isomers, HPCE)

Synonym(s):

Atto 488 - Nitrilotriacetic acid, complexed to Ni2+ - ion, Nα,Nα-bis(carboxymethyl)-L-lysine, Nickel(II) complex

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About This Item

UNSPSC Code:
12171501
NACRES:
NA.32

product line

BioReagent

Assay

≥80% (sum of isomers, HPCE)

form

solid

fluorescence

λex 501 nm; λem 523 nm in PBS with 0.08% sodium azide

suitability

suitable for fluorescence

detection method

fluorometric

storage temp.

−20°C

General description

find more information here

Application

for the fluorescence detection of HIS-tagged proteins

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Pictograms

Health hazardEnvironment

Signal Word

Danger

Hazard Classifications

Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 1A - Muta. 2 - Repr. 1A - Resp. Sens. 1 - Skin Sens. 1 - STOT RE 1 Inhalation

Storage Class Code

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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The changing point-spread function. Single-molecule-based super-resolution imaging.
Horrocks, M. H.; Palayret, M.; Klenerman, D.; Lee, S. F.
Histochemistry and Cell Biology, 141(6), 577-585 (2014)
Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging.
van de Linde, S.; et al.
Photochemistry and Photobiology, 10(4), 499-506 (2011)
Verbesserte hochauflosende Mikroskopie mit Oxazinfarbstoffen in schwerem Wasser.
Lee, S. F.; Verolet, Q.; Furstenberg, A.
Angewandte Chemie (International Edition in English), 124(34), 9117-9120 (2013)
Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy.
Vogelsang, J.; et al.
Proceedings of the National Academy of Sciences of the USA, 106(20), 8107-8112 (2009)
Intrinsically Resolution Enhancing Probes for Confocal Microscopy.
Vogelsang, J.; et al.
Nano Letters, 10(2), 672-679 (2010)

Articles

Ni-NTA-Atto conjugates provide specific and highly sensitive detection of His-tagged fusion proteins.

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