Skip to Content
Merck
All Photos(4)

Documents

151173

Sigma-Aldrich

Ninhydrin

ACS reagent

Synonym(s):

1,2,3-Indantrione monohydrate, 2,2-Dihydroxy-1,3-indanedione, Trioxohydrindene monohydrate

Sign Into View Organizational & Contract Pricing
All Photos(4)

About This Item

Empirical Formula (Hill Notation):
C9H6O4
CAS Number:
485-47-2
Molecular Weight:
178.14
Beilstein:
1910963
EC Number:
207-618-1
MDL number:
MFCD00003791
UNSPSC Code:
12352100
PubChem Substance ID:
24849098
NACRES:
NA.21

grade

ACS reagent

Quality Level

200

description

identification and melting point passes test

form

solid

color

white to brownish white, crystals

mp

250 °C (dec.) (lit.)

solubility

H2O: passes test

suitability

passes test for sensitivity to amino acids

SMILES string

OC1(O)C(=O)c2ccccc2C1=O

InChI

1S/C9H6O4/c10-7-5-3-1-2-4-6(5)8(11)9(7,12)13/h1-4,12-13H

InChI key

FEMOMIGRRWSMCU-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

General description

Ninhydrin, a tricyclic 1,2,3-trione, is a useful organic building block. It is an amino acid reagent, which reveals latent fingerprints on porous surfaces like paper, cardboard and raw wood.
It is employed for nitrite detection in various samples, identifying contamination, and is also used as a colorimetric reagent.

Application

Ninhydrin (Triketohydrindene hydrate) may be used as a reagent for the quantification of amino acids and peptides by photometric method and colorimetric method. It may be used to prepare the ninhydrin reagent, by mixing ninhydrin and hydrindantin (reduced form of ninhydrin) in dimethyl sulfoxide. This reagent is used in manual ninhydrin method for the quantitative determination of amino acids.
Used in the detection and assay of peptides, amino acids, amines, and amino sugars yielding highly fluorescent ternary compounds with aldehydes and primary amines. Reaction with sarcosine or proline gives azomethine ylides.
Used for the detection of free amino groups in amino acids, peptides and proteins.

Pictograms

Exclamation mark
GHS07

Signal Word

Warning

Hazard Statements

H302,H315,H319

Hazard Classifications

Acute Tox. 4 Oral - Eye Irrit. 2 - Skin Irrit. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000403082
0000403081
0000372772
0000325470
0000403081

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Gazzetta Chimica Italiana, 123, 137-137 (1993)
Holden.MH
To Be a Crime Scene Investigator (2006)
Shao-Ru Wang et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 7(13), 1903770-1903770 (2020-07-17)
CRISPR (clustered regularly interspaced short palindromic repeats) systems have been established as valuable genome-editing tools. Controlling CRISPR systems has high biological significance and this field has garnered intense interest. There is a considerable need for simple approaches with no need
Tetrahedron, 34, 1285-1285 (1978)
E Curotto et al.
Analytical biochemistry, 211(2), 240-241 (1993-06-01)
Chitosans were quantified with ninhydrin, a reagent normally used for recognizing and quantifying amino groups. The reaction was time dependent and there was no effect when different acids were added to the mixture. This method was used to determine the
View All Related Papers

Articles

Determination of Sialic Acid in Milk

Discover the detection of milk adulteration by whey using spectrophotometric estimation of sialic acid in three different milk brands. Learn more about this essential technique in our application note.

Determination of Sialic Acid in Milk

Discover the detection of milk adulteration by whey using spectrophotometric estimation of sialic acid in three different milk brands. Learn more about this essential technique in our application note.

Determination of Sialic Acid in Milk

Discover the detection of milk adulteration by whey using spectrophotometric estimation of sialic acid in three different milk brands. Learn more about this essential technique in our application note.

Determination of Sialic Acid in Milk

Discover the detection of milk adulteration by whey using spectrophotometric estimation of sialic acid in three different milk brands. Learn more about this essential technique in our application note.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service