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MAB1275

Sigma-Aldrich

Anti-HLA-ABC Common Antibody, clone 22.63.4

clone 22.63.4, Chemicon®, from mouse

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified antibody

antibody product type

primary antibodies

clone

22.63.4, monoclonal

species reactivity

human

manufacturer/tradename

Chemicon®

technique(s)

flow cytometry: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable

isotype

IgG2b

NCBI accession no.

UniProt accession no.

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HLA-A(3105)

Related Categories

General description

BIOCHEMISTRY: p I = 7.8
The previously assigned protein identifier P16189 has been merged into P04439. Full details can be found on the UniProt database.

Specificity

This antibody reacts with a common framework determinant of the 40-44kD protein of HLA-ABC Class I antigen. The antibody precipitates antigens of 40 - 44kD and 12kD (b2-microglobulin).

Cell reactivity:

The antibody reacts with all cells having HLA-ABC surface antigen. Erythrocytes and K562 and DAUDI are negative.

Immunogen

Nalm-6 cell line

Application

Research Category
Inflammation & Immunology
Research Sub Category
Immunoglobulins & Immunology
This Anti-HLA-ABC Common Antibody, clone 22.63.4 is validated for use in FC, IC, IH for the detection of HLA-ABC.
This antibody is suitable for indirect immunofluorescence analysis by flow cytometry and is an excellent positive control. It is suitable for cryostat sections but not for routine formalin fixed paraffin sections. If the tissue is prepared at 4°C and fixed in PLP (Periodate-Lysine-Paraformaldehyde) before paraffin embedding, the antigen can be conserved (Hancock, 1982).

SUGGESTED USAGE DILUTION

1. Flow cytometry and indirect immunofluorescence 1:15 - 1:60

Dilute with isotonic buffer. Use 50 μl of diluted antibody per 1 x 10E6 peripheral blood mononuclear cells (PBMC) in 100 μl buffer.

2. Indirect immunoperoxidase staining - the final dilution will depend on the assay conditions and detection system employed. However, a dilution of at least 1:15 will be applicable to most commonly used systems.

Linkage

Replaces: MAB1280

Physical form

Format: Purified
Purified from mouse ascitic fluid. The antibody is supplied in phosphate buffered saline, pH 7.4 containing 0.1% sodium azide, diluted 50% v/v with glycerol. The characteristics of each lot are tested by electrophoresis and flow cytometry.

Storage and Stability

Store at 2 to 8°C, for up to 6 months. For prolonged periods, store at -15 to -20°C in undiluted aliquots. At this temperature the glycerol solution will not freeze.

WARNING: The monoclonal reagent solution contains 0.05% sodium azide as a preservative. Due to potential hazards arising from the build up of this material in pipes, spent reagent should be disposed of with liberal volumes of water.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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