XNAB2R
Extract-N-Amp™ Blood PCR Kit
sufficient for 1000 extractions, sufficient for 1000 amplifications
Synonym(s):
Blood direct PCR kit
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About This Item
UNSPSC Code:
41106303
NACRES:
NA.55
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usage
sufficient for 1000 amplifications
sufficient for 1000 extractions
sufficient for 1000 reactions
Quality Level
feature
dNTPs included
hotstart
technique(s)
PCR: suitable
color
colorless
shipped in
wet ice
storage temp.
−20°C
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General description
The Extract-N-Amp™ Blood PCR Kits contain all of the reagents necessary to rapidly extract host genomic DNA from whole blood and amplify targets of interest by direct PCR. This novel extraction system eliminates the need for any type of purification, organic extraction, centrifugation, heating, filtration or alcohol precipitation. The kit also includes a PCR ReadyMix™, specially formulated for amplification directly from the extract. This formulation uses an antibody based hot start, for specific amplification.
Application
Extract-N-Amp™ Blood PCR Kit has been used to extract DNA from genomes and dried blood spot samples. It has also been used for polymerase chain reaction (PCR).
Features and Benefits
- Efficient 8 minute prep allows greater speed and throughput
- No need for any type of purification, organic extraction, centrifugation or alcohol precipitation
- Simple, 3 step procedure with no special equipment required
- Hot start antibody included for highly specific PCR amplification of genomic DNA
- Compatible with multiple format (single tube or 96-well plate)
- Can be used with whole blood or blood cards
- Extract stable at 4 °C for at least 6 months
Principle
Genomic DNA is extracted from 10 μl of whole blood by simply adding the Extraction Solution and incubating for 5 minutes at room temperature. The Neutralization Solution is added to the extract to counteract inhibitory substances prior to PCR. A portion of the DNA extract is then added to the specially formulated PCR ReadyMix.
Other Notes
For additional information, please see www.sigma-aldrich.com/extract-n-amp.
Legal Information
Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser′s own internal research. No other patent rights (such as 5′ Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Antibody licensed for in vitro research use under U.S. Patent No. 5,338,671 and 5,587,287, and corresponding patents in other countries.
Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
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Storage Class Code
10 - Combustible liquids
Certificates of Analysis (COA)
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Nicholas Wong et al.
BioTechniques, 45(4), 423-424 (2008-10-16)
Sodium bisulfite treatment followed by PCR and DNA sequencing is widely considered the gold standard for the analysis of DNA methylation patterns. However, this technique generally requires substantial quantities of genomic DNA as starting material and is often associated with
Mads V Hollegaard et al.
BMC genomics, 10, 297-297 (2009-07-07)
Identification of disease susceptible genes requires access to DNA from numerous well-characterised subjects. Archived residual dried blood spot samples from national newborn screening programs may provide DNA from entire populations and medical registries the corresponding clinical information. The amount of
Jun Shi et al.
Cancer research, 67(13), 6417-6424 (2007-07-10)
Idiopathic myelofibrosis (IM) is likely the consequence of both the acquisition of genetic mutations and epigenetic changes that silence critical genes that control cell proliferation, differentiation, and apoptosis. We have explored the effects of the sequential treatment with the DNA
Mads Vilhelm Hollegaard et al.
Electrophoresis, 30(14), 2532-2535 (2009-07-30)
Stored surplus of dried blood spot (DBS) samples from neonatal screening programs constitute a vast potential for large genetic epidemiological studies. However, age of the samples and the small amounts of DNA available may limit their usage. In this study
Sandrine Romand et al.
Biotechnology and bioengineering, 113(5), 1094-1101 (2015-11-03)
Chinese Hamster Ovary (CHO) cells are widely used for the large-scale production of recombinant biopharmaceuticals. However, attempts to express IGF-1 (a mutated human Insulin-like growth factor 1 Ea peptide (hIGF-1Ea mut)) in CHO cells resulted in poor cell growth and
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