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T7902

Thermolysin from Geobacillus stearothermophilus

powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

Synonym(s):

Protease from Geobacillus stearothermophilus, Thermophilic-bacterial protease

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About This Item

CAS Number:
9073-78-3
UNSPSC Code:
12352204
NACRES:
NA.75
EC Number:
232-973-4
MDL number:
MFCD00165549
EC Number:
3.4.24.27 (BRENDA, IUBMB)
eCl@ss:
32160410
Specific activity:
30-350 units/mg protein (E1%/280)
Biological source:
Geobacillus stearothermophilus
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biological source

Geobacillus stearothermophilus

Quality Level

200

product line

BioReagent

form

powder

specific activity

30-350 units/mg protein (E1%/280)

mol wt

34.6 kDa

purified by

crystallization

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

storage temp.

−20°C

Application

A thermostable (thermophilic) extracellular metalloendopeptidase containing four calcium ions. Cofactors are zinc and calcium. Hydrolyzes protein bonds on the N-terminal side of hydrophobic amino acid residues. The pH optimum is 8.0 and the optimal temperature for activity is 70 °C. Considerably stable from pH 5 to 9.5. Thermolysin has a low cleavage specificity, therefore, it produces a number of short fragments that are suitable for sequencing. Preferential cleavage: X-cleavage-Y-Z where X=any amino acid; Y=Leu, Phe, Ile, Val, Met, Ala and Z is any amino acid other than Pro. Cleavage N-terminal to Leu is preferred over cleavage of N-terminal to Phe which is preferred over the others. Often used to do limited proteolysis for peptide mapping and studies of protein structure and conformational changes.

Physical form

lyophilized powder containing calcium and sodium acetate buffer salts

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).

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This Item
P1512C9722T9935
Thermolysin from Geobacillus stearothermophilus powder, BioReagent, 30-350 units/mg protein (E1%/280), suitable for cell culture

Sigma-Aldrich

T7902

Thermolysin from Geobacillus stearothermophilus

Thermolysin from Geobacillus stearothermophilus Type X, lyophilized powder, 30-350 units/mg protein (E1%/280)

Sigma-Aldrich

P1512

Thermolysin from Geobacillus stearothermophilus

Collagenase from Clostridium histolyticum lyophilized powder (from 0.2μm filtered solution), 0.5-5.0 FALGPA units/mg solid, suitable for cell culture

Sigma-Aldrich

C9722

Collagenase from Clostridium histolyticum

Trypsin from bovine pancreas essentially salt-free, lyophilized powder, ≥9,000 BAEE units/mg protein, BioReagent, suitable for cell culture

Sigma-Aldrich

T9935

Trypsin from bovine pancreas

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

specific activity

30-350 units/mg protein (E1%/280)

specific activity

30-350 units/mg protein (E1%/280)

specific activity

0.5-5.0 FALGPA units/mg solid

specific activity

≥9,000 BAEE units/mg protein

biological source

Geobacillus stearothermophilus

biological source

Geobacillus stearothermophilus

biological source

Clostridium histolyticum

biological source

-

form

powder

form

lyophilized powder

form

lyophilized powder (from 0.2μm filtered solution)

form

essentially salt-free, lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

mol wt

34.6 kDa

mol wt

34.6 kDa by amino acid sequence

mol wt

-

mol wt

23.8 kDa

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signalword

Danger

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves

target_organs

Respiratory system

pictograms

Health hazardExclamation mark
GHS08,GHS07

Hazard Classifications

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

wgk

WGK 1

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Certificates of Analysis (COA)

Lot/Batch Number
0000550089
0000540308
0000531279
0000531277
0000531278

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Allen F Ryan et al.
Frontiers in genetics, 11, 358-358 (2020-05-01)
Single-cell transcriptomics was used to profile cells of the normal murine middle ear. Clustering analysis of 6770 transcriptomes identified 17 cell clusters corresponding to distinct cell types: five epithelial, three stromal, three lymphocyte, two monocyte, two endothelial, one pericyte and
Lukas Menges et al.
British journal of pharmacology, 176(24), 4696-4707 (2019-08-20)
The intracellular signalling molecule cGMP, formed by NO-sensitive GC (NO-GC), has an established function in the vascular system. Despite numerous reports about NO-induced cGMP effects in the heart, the underlying cGMP signals are poorly characterized. Therefore, we analysed cGMP signals
David P Minde et al.
PloS one, 7(10), e46147-e46147 (2012-10-12)
The biophysical stability is an important parameter for protein activity both in vivo and in vitro. Here we propose a method to analyse thermal melting of protein domains in lysates: Fast parallel proteolysis (FASTpp). Combining unfolding by a temperature gradient
View All Related Papers

Global Trade Item Number

SKUGTIN
T7902-25MG04061837378737
T7902-250MG04061837378720
T7902-100MG04061837378713

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