T5192

Anti-γ-Tubulin antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• Synonym(s): Anti-γ-Tubulin
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 48 kDa
• species reactivity: human, chicken
• packaging: antibody small pack of 25 μL
• enhanced validation: independent; Learn more about Antibody Enhanced Validation
• technique(s): indirect immunofluorescence: 1:1,000 using methanol/acetone-fixed chicken fibroblasts cells; western blot: 1:1,000 using whole cell extract human epidermal carcinoma A431 cell line
• UniProt accession no.: P23258
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... TUBG1(7283) , TUBG2(27175)

Description

General description

γ-Tubulin, mapped on human chromosome 17q21.2, codes for a member of the tubulin family. It is localized to the microtubule organizing centres. γ-Tubulin consists of two isoforms tubulin γ 1 (TUBG1) and tubulinγ 2 (TUBG2) with 97.3% amino acid identity. In addition to these two isoforms, γ-tubulin pseudogene (TUBG1P) is also been identified on human chromosome 17. Human TUBG1 and TUBG2 transcripts are widely expressed in preimplantation embryos and the brain, respectively. γ-tubulin is a component of γ-tubulin ring complex (γ-TuRC), which has roughly the same diameter as a microtubule. γ-Tubulin does not polymerize with α-tubulin/β-tubulin, but instead it is localized to the centrosome and to the cytoplasm.

Immunogen

synthetic peptide corresponding to the N-terminal region of human γ-tubulin (amino acids 38-53, with C-terminally added lysine) conjugated to KLH. This sequence is specific for γ-tubulin and not found in other members of the tubulin family such as α− β−, δ and ε− tubulins. This sequence is identical in mouse and rat γ-tubulin and highly conserved among species (Drosophila, Aspergillus, and yeast γ-tubulin).

Application

Anti-γ-Tubulin antibody produced in rabbit has been used in following studies:

• Immunocytochemistry.
• Immunofluorescence.
• Immunoblotting.
• Staining in microscopy.
• Chromatin immunoprecipitation (ChIP).

Biochem/physiol Actions

γ-Tubulin nucleates microtubule assembly throughout the mammalian cell cycle in vivo. In Aspergillus nidulans, γ-tubulin facilitates attachment of microtubules to the spindle pole body, nuclear division and microtubule assembly. Ubiquitination of γ-tubulin by breast cancer 1 protein (BRCA1) is a crucial step in the regulation of centrosome number. Overexpression of γ-tubulin is observed in lung cancer. Tubulin γ 2 (TUBG2) plays a vital role in cell growth. Aberration in the γ-tubulin gene alters microtubule assembly. The expression levels of γ-tubulin can be considered as an important prognostic indicator for patients with astrocytomas.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable