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T3824

Sigma-Aldrich

Tyrosinase from mushroom

Tyrosinase from mushroom

lyophilized powder, ≥1000 unit/mg solid

Synonym(s):

Catechol Oxidase, Monophenol Monooxygenase, Monophenol, dihydroxyphenylalanine:oxygen oxidoreductase, Polyphenol Oxidase

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About This Item

CAS Number:
9002-10-2
Enzyme Commission number:
1.14.18.1 (BRENDA, IUBMB)
MDL number:
MFCD00082118
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

biological source

fungus (mushroom)

Quality Level

200

form

lyophilized powder

specific activity

≥1000 unit/mg solid

mol wt

119.5 kDa by electrophoresis
 128 kDa by sedimentation velocity diffusion
 133 kDa by LS

application(s)

diagnostic assay manufacturing

shipped in

wet ice

storage temp.

−20°C

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General description

Isoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

Tyrosinase is a copper-containing oxidase, which has activity for both catechols and cresol. It is responsible for browning reactions. The enzyme is reported to have two binding sites for aromatic substrates and a different binding site for oxygen-copper. The copper is probably Cu(I), with inactivation involving oxidation to Cu(II) ion.
Tyrosinase is a copper-containing oxidase with activity for catechols and cresol. Tyrosinase participates in the conversion of tyrosine to dihydroxyphenylalanine (DOPA), and polymerization of DOPA leads to melanin formation. Tyrosinase has a role in browning reactions. The enzyme is said to have two binding sites for aromatic substrates and a distinct binding site for oxygen-copper.

Application

Tyrosinase activity has been assessed in a study that developed an alternative therapeutic agent for treating hyperpigmentation. [1] Tyrosinase has also been used in a study to investigate the oculocutaneous albinism phenotype in the Pakistani population. [2]

Biochem/physiol Actions

Converts tyrosine to L-DOPA or tyramine to dopamine

Physical properties

Isoelectric point (pI): 4.7-5
pH optimum is 6-7

Molecular weight: 128 kDa by sedimentation velocity diffusion; 133 kDa by light-scattering measurements, and 119.5 kDa by electrophoresis.

Unit Definition

One unit = ΔA280 of 0.001 per min at pH 6.5 at 25 °C in 3 mL reaction mix containing L-tyrosine.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
0000407328
0000396734
0000396735
0000391635
0000387758

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If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

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A T S Jorge et al.
International journal of cosmetic science, 34(5), 435-440 (2012-06-29)
Several treatments for skin whitening are available today, but few of them are completely adequate, especially owing to the carcinogenic potential attributed to classical drugs like hydroquinone, arbutin and kojic acid. To provide an alternative and safer technology for whitening
Shilpi Goenka et al.
Molecules (Basel, Switzerland), 25(16) (2020-08-13)
Previous studies have reported that estrogen hormone promotes melanogenesis while progesterone inhibits it. A selective estrogen receptor modulator (SERM), tamoxifen, has been shown to promote melanogenesis; however, to date, there have been no reports on the effects of a selective
Tyrosinase inhibitory effects and inhibition mechanisms of nobiletin and hesperidin from citrus peel crude extracts.
Zhang, et al.
Journal of Enzyme Inhibition and Medicinal Chemistry, 22, 83-90 (2019)
Rongchao Zhang et al.
Evidence-based complementary and alternative medicine : eCAM, 2020, 2582579-2582579 (2020-08-11)
Whitening cosmetics market has a bright future, and pure natural whitening products of traditional Chinese medicine have always been a research hotspot. In this research, the whitening active ingredient of Chinese medicine Trichosanthes pulp was isolated and purified for the
Thomas J Jaworek et al.
Orphanet journal of rare diseases, 7, 44-44 (2012-06-28)
Oculocutaneous albinism (OCA) is caused by a group of genetically heterogeneous inherited defects that result in the loss of pigmentation in the eyes, skin and hair. Mutations in the TYR, OCA2, TYRP1 and SLC45A2 genes have been shown to cause
View All Related Papers
2 Reviews This action will navigate to reviews.

Questions

1–9 of 9 Questions  

  1. Is this tyrosinase is derived from Agaricus bisporus or another mushroom species?

    1 answer

    1. Yes, this product is derived from Agaricus bisporus.

      Helpful?

  2. Can you please tell me 25000 units tyrosinase in milligrams?

    1 answer

    1. This product is sold by activity. The minimum activity specification for this material is 1000 units per milligram. The weight of this product can be determined using the activity value reported on the lot specific Certificate of Analysis(COA). For example, the sample COA has a activity value of 6540 units/mg. The amount of powder in the 25,000 unit package will be 3.82mg.

      Please see the link below or navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot specific Certificate:
      https://www.sigmaaldrich.com/US/en/product/sigma/t3824#product-documentation

      Helpful?

  3. Hi, How much is the total protein amount of T3824 (25KU) per vial?

    1 answer

    1. The protein content of this product is not determined. This product is tested and released based on the activity per milligram solid. Should the protein content be required, please see the link below to review several kits that may be of interest:
      https://www.sigmaaldrich.com/US/en/product/compare?term=total%20protein%20kit&facet%5B0%5D=facet_product_category%3Akits&focus=products&page=1&perpage=30&sort=relevance&type=product&products=TP0300%3ASIGMA%3Asial%2CTP0200%3ASIGMA%3Asial%2CTP0100%3ASIGMA%3Asial

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  4. What solution is best to be used to reconstitute the T3824-25KU? and what is the volume and concetration of that soluation?

    1 answer

    1. Tyrosinase is soluble at 2 mg/mL in 50 mM potassium phosphate buffer. Please see the link below to review the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/377/683/t3824dat-ms.pdf

      Helpful?

  5. I used to order another tyrosinase (Product No. T7755), which has since been discontinued. What is the difference between the replacement product T3824 and the discontinued product T7755?

    1 answer

    1. The difference between these two products is that T3824 has been stabilized with trehalose, while T7755 was not.

      Helpful?

  6. I see that the approximate activity of Product T3824, Tyrosinase from mushroom, is  ?1000 unit/mg solid, but how can I find the lot specific activity of this tyrosinase?

    1 answer

    1. The certificate of analysis for the lot will report the exact tyrosinase activity for a particular batch. The lot number can be found on the label of the bottle, or you can call technical service (800-325-5832) for a sample lot number to view the online certificate of analysis.

      Helpful?

  7. How can Tyrosinase from mushroom, Product T3824, be dissolved and what is its expected stability in solution?

    1 answer

    1. Tyrosinase can be dissolved in 50 mM potassium phosphate buffer at 2 mg/mL.  Solutions are expected to retain activity for several days in the refrigerator (2-8°C) and for several weeks in the freezer (-20°C).

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  8. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  9. The website says Product T3824, Tyrosinase from mushroom, should be stored at -20°C, but I received it at room temperature. Is this correct?

    1 answer

    1. The product is shipped at ambient temperature and is stable for up to two weeks during shipment.  Once received, it is recommended to store the product at -20°C to insure the quality for long term storage.

      Helpful?

Reviews

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  1. Madrid, Spain
    • Review 1
    • Votes 0
    5 out of 5 stars.

    Tyrosinase from mushroom

    All the products arrived in due time and storage conditions.

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  2. 1 Ratings-Only Review

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