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SAB5900202

Sigma-Aldrich

Alpaca Anti-PD-L1, recombinant single domain Antibody

Recombinant Alpaca(VHH) Nano antibody

Synonym(s):

B7-H, B7H1, PD-L1, PDCD1LG1, PDL1

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.43

biological source

alpaca

Quality Level

recombinant

expressed in E. coli

conjugate

unconjugated

antibody form

affinity purified VHH antibody

clone

PL03, monoclonal

Assay

>95% (SDS-PAGE)

form

lyophilized solid

mol wt

14.4 kDa
~ 15-18 kDa (SDS PAGE under reducing condition)

purified by

immobilized metal affinity chromatography (IMAC)

species reactivity

human, (Cynomolgus macaques)

concentration

1 mg/mL

technique(s)

ELISA: 1:10000-1:100000
flow cytometry: 1:50-1:100
immunofluorescence: 1:200-1:800
immunohistochemistry: 1:200-1:800
western blot: 1:1000-1:3000

color

colorless

pH

( in PBS, pH 7.4)

isotype

VHH

Protein ID accession no.

UniProt accession no.

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Gene Information

human ... CD274(29126)

General description

PD-L1, also known as CD274 or B7H1, stands for programmed cell death ligand 1. It is a type I transmembrane protein that is thought to repress immune responses by binding to its receptor (PD1), thus inhibiting T-cell activation, proliferation, and cytokine production. It contains V-like and C-like immunoglobulin domains. PD-L1 expression is regulated by various cytokines, such as TNF-α or LPS (ISSN: 1848-7718). Increased expression of this protein in certain types of cancers, e.g., renal cell carcinoma or colon cancer, correlates with poor prognosis.Alpaca derived anti-PD-L1 VHH single domain antibody (molecular weight: 14.4kDa), expressed in E. coli under conditions free from animal derived components. It has a 6His tag at its C-terminal.
Single-domain antibody (sdAb), also called nano-antibody, is the variable region isolated from the antigen-binding variable domain (VHH) of camelid heavy-chain-only antibody. It lacks light chains and a first constant domain(CH1). This configuration confers a number of unique advantages over conventional monoclonal antibodies. The small size of just 15 kDa (2~4 nm), single-domain antibody is the smallest antigen-binding fragment that can easily penetrate tissues, allowing them to bind epitopes that are inacessible to conventional antibodies. The small size and monovalency makes it a superior tool in microsopy. These single-domain antibodies offer superior chemical (wide variety of pH, urea, etc) and thermal stability and increased solubility than conventional antibodies. All our antibodies are tested to ensure the highest level of specificity, sensitivity and reproducibility.

Specificity

Human Programmed Death-Ligand 1 (PD-L1)

Immunogen

Recombinant human PD-L1 (Phe19-Tyr134) protein

Application

ELISA, WB, FC, IHC, IF

Packaging

50 µg per vial / 100 µg per vial

Physical form

Affinity purified VHH single domain antibody lyophilized from PBS, pH7.5

Reconstitution

Reconstitute the lyophilized antibody with sterile deionized water or with 50% glycerol (ACS grade or better) to a stock solution of 1 mg/mL. Solubilize for 30 to 60 minutes at room temperature with occasional gentle mixing. Avoid vigorous shaking or vortexing.

Storage and Stability

Stable for 1 year at 4°C from date of receipt.

Other Notes

Storage at 4°C; Do not freeze.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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