S6402

Streptavidin−Cy3^™ from Streptomyces avidinii

buffered aqueous solution

• Synonym(s): Streptavidin Cy3
• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00164141

Properties

• conjugate: CY3 conjugate
• Quality Segment: 200
• form: buffered aqueous solution
• extent of labeling: 3-9 mol fluorochrome per mol protein
• technique(s): immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:100
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Streptavidin is a homotetramer protein. One molecule of streptavidin binds four molecules of biotin by a non-covalent interaction that is essentially irreversible. Streptavidin has a high affinity for biotin and is extensively used as a labeling tool and for studies involving binding proteins.

Application

Streptavidin−Cy3^™ from Streptomyces avidinii has been used:

• in double immunofluorescence staining to detect biotinylated lectin
• for insulin and C-peptide staining in immunofluorescence analysis
• in double immunofluorescence to verify the presence of cells filled with biocytin
• in conjunction with biotinylated antibodies in immunohistochemistry and immunocytochemistry
• with the traditional rhodamine filter arrangements due to the spectral overlap

Features and Benefits

Spectral Characteristics of Cy3 Dye:

• Absorbance Max 552 nm
• Emission Max 565 nm
• Molar Extinction Coefficient 1.3 x 10^-5 M^-1cm^-1

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin, and 15 mM sodium azide

Legal Information

Cy3 is a trademark of Cytiva

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• PPE (personal protective equipment): Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)