S1797
SOC Medium
For use in transformation
Synonym(s):
Super Optimal broth
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About This Item
UNSPSC Code:
41106200
NACRES:
NA.85
Recommended Products
grade
for molecular biology
Quality Level
sterility
0.2 μm filtered
form
liquid
composition
Dextrose, 3.603 g/L
KCl, 0.186 g/L
MgSO4, 4.8 g/L
Tryptone, 20 g/L
Yeast extract, 5 g/L
technique(s)
microbiological culture: suitable
application(s)
agriculture
storage temp.
2-8°C
suitability
nonselective for Escherichia coli
nonselective for coliforms
General description
Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.
Application
SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.
Features and Benefits
SOC liquid provides:
- Filter-sterilized, ready-to-use format
- Convenient package sizes
- Standard formulation
related product
Product No.
Description
Pricing
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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In this paper, we report a useful protocol for cloning toxic protein genes. Use of the SOC medium, which is a glucose-containing rich medium, significantly improved the transformation efficiency of a recombinant plasmid containing a toxic plant subtilase SaSBT1 cDNA.
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