RAB0026

Human BDNF ELISA Kit

for serum, plasma, cell culture supernatant and urine

• Synonym(s): BDNF, Brain-derived neurotrophic factor
• NACRES: NA.32
• UNSPSC Code: 41116158
• Gene information: human ... BDNF(627)
• Input: sample type plasma; sample type serum; sample type cell culture supernatant(s); sample type urine
• Species reactivity: human
• Storage temp.: −20°C
• Shipped in: wet ice

Properties

• species reactivity: human
• packaging: kit of 96 wells (12 strips x 8 wells)
• technique(s): ELISA: suitable, capture ELISA: suitable
• input: sample type plasma; sample type serum; sample type cell culture supernatant(s); sample type urine
• assay range: inter-assay cv: <12%; intra-assay cv: <10%; sensitivity: 80 pg/mL; standard curve range: 0.066-16 ng/mL
• detection method: colorimetric
• shipped in: wet ice
• storage temp.: −20°C
• Gene Information: human ... BDNF(627)

Description

General description

The Human BDNF ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human BDNF in serum, plasma, cell culture supernatants and urine.

The brain derived neurotrophic factor (BDNF) gene is mapped to human chromosome 11p14.1.[1] BDNF is a member of the neurotrophin family of growth factors.[2] The gene encodes a precursor protein, proBDNF. Mature BDNF (mBDNF) is synthesized by post-translational cleavage of proBDNF. Both proBDNF and mBDNF play crucial roles in cellular signaling.[3]

Immunogen

Recombinant Human BDNF

Application

For research use only. Not for use in diagnostic procedures.
Human BDNF ELISA kit has been used to determine the effect of bone marrow mesenchymal stem cell (MSC) transplantation on BDNF protein expression in the striatum of Tourette syndrome (TS) rats.[4] It has also been used to determine the plasma BDNF levels in GD1 (Gaucher′s disease type 1) patients and control population.[5]

Human BDNF ELISA Kit has been used:

• to measure the brain-derived neurotrophic factor (BDNF) protein expression
• to determine BDNF level in the slice culture medium and the extracts of organotypic brain slice cultures (OBSCs)
• to determine BDNF level in plasma

Biochem/physiol Actions

Brain derived neurotrophic factor (BDNF) is involved in hippocampal function and verbal episodic memory in humans. Hence, variation in the BDNF gene expression alters these neurological functions.[6] BDNF also plays a vital role in vascular function and participates in angiogenesis via the specific receptor tropomyosin-related kinase B (TrkB).[7] It is involved in the pathogenesis of Alzheimer′s disease. ProBDNF interacts with p75 neurotrophin receptor, leading to long-term depression in the hippocampus.[3]

Other Notes

A sample Certificate of Analysis is available for this product.
Please type the word sample in the text box provided for lot number.

Kit Components Also Available Separately

• RABELADA

  ELISA 1X Assay/Sample Diluent Buffer A (Item D1)

  SDS
• RABELADB

  ELISA 5X Assay/Sample Diluent Buffer B (Item E1)

  SDS
• RABSTOP3

  ELISA Stop Solution (Item I)

  SDS
• RABTMB3

  ELISA Colorimetric TMB Reagent (HRP Substrate, Item H)

  SDS
• RABWASH4

  20X Wash Buffer (Item B)

  SDS

Safety Information

• pictograms: GHS05
• signalword: Warning
• hcodes: H290
• pcodes: P234 - P390
• Hazard Classifications: Met. Corr. 1
• Storage Class: 10 - Combustible liquids