QR0100
SYBR® Green Quantitative RT-qPCR Kit
One step SYBR® Green RT-qPCR with MMLV & hot-start Taq DNA Polymerase
Synonym(s):
one step rt qPCR, sybr green qPCR
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About This Item
UNSPSC Code:
41106300
NACRES:
NA.55
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usage
sufficient for 250 reactions
feature
dNTPs included
hotstart
technique(s)
RT-qPCR: suitable
color
colorless
input
purified RNA
compatibility
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detection method
SYBR® Green
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
The SYBR® Green Quantitative RT-PCR Kit combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT), JumpStart™ Taq DNA polymerase, and SYBR Green I fluorescent dye in a one-step RT-PCR kit designed for measurement of gene expression. This convenient 2X ready mix includes M-MLV RT, SYBR Green I dye, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques.
Application
SYBR® Green Quantitative RT-qPCR Kit has been used in a 1-step reverse transcription polymerase chain reaction (RT-PCR) assay:
- to detect specific genetic clusters of genogroup I and II noroviruses
- for chikungunya viral (CHIKV) RNA quantification
- to detect mRNA expression levels
- for amplification of total RNA extracted from human umbilical vein endothelial cells (HUVECs) and prostate cancer cells
Features and Benefits
- The master mix allows consistency and reproducibility from one reaction to the next
- Reduced preparation time and the risk of contamination from multiple pipetting steps
- Reduced set-up time as compared to manual or wax Hot Start methods
- JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation
- SYBR® Green I dye is inexpensive, easy to use, and highly sensitive
- Broad instrument compatibility
- Includes a separate ROX reference dye vial for reaction normalization
Packaging
1 kit sufficient for 100 reactions at 50 μL each
Legal Information
Use of this product is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,994,056 and 6,171,785.. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product for the purchaser′s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) and no right to perform commercial services of any kind, including without limitation reporting the results of purchaser′s activities for a fee or other commercial consideration, is conveyed expressly, by implication, or by estoppel. This product is for research use only. Diagnostic uses under Roche patents require a separate license from Roche. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA.
Eppendorf is a registered trademark of Eppendorf AG
JumpStart is a trademark of Sigma-Aldrich Co. LLC
SYBR is a registered trademark of Life Technologies
Kit Components Only
Product No.
Description
- SYBR® Green Taq ReadyMix™ for Quantitative RT-PCR 2 x 25
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1C - Skin Sens. 1
Storage Class Code
8A - Combustible corrosive hazardous materials
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Genogroup I noroviruses from five genetic clusters and genogroup II noroviruses from eight genetic clusters were detected in stool extracts using degenerate primers and single-tube, real-time reverse transcription-PCR (RT-PCR) with SYBR Green detection. Two degenerate primer sets, designated MON 431-433
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