P6662
o-Phenylenediamine dihydrochloride
tablet, 1 mg substrate per tablet
Synonym(s):
1,2-Phenylenediamine tablet, OPD, OPD Tablet
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About This Item
Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
Molecular Weight:
181.06
Beilstein:
3912045
MDL number:
UNSPSC Code:
12352204
PubChem Substance ID:
NACRES:
NA.83
Recommended Products
Quality Level
form
tablet
mp
258 °C (dec.) (lit.)
solubility
water: 1 tablet/10 mL, clear, colorless
storage temp.
2-8°C
SMILES string
Cl[H].Cl[H].Nc1ccccc1N
InChI
1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
InChI key
RIIWUGSYXOBDMC-UHFFFAOYSA-N
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General description
o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.
Reconstitution
Dissolve one tablet in 0.05 M phosphate-citrate buffer, pH 5.0, to the desired concentration (typically an OPD concentration of 0.4 mg/ml is used). Add 40 μl of fresh 30% hydrogen peroxide per 100 ml of substrate buffer solution, immediately prior to use. Tablets are individually packaged in foil packets.
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Muta. 2 - Skin Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
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