Skip to Content
Merck
All Photos(2)

Key Documents

P1253

Sigma-Aldrich

Phosvitin from egg yolk

Sign Into View Organizational & Contract Pricing


About This Item

CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.81

biological source

egg yolk

Quality Level

form

powder

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

General description

Phosvitin from egg yolk is a polyanionic phosphoserine rich protein. This phosphoglycoprotein comprises α-helices and β-sheets structural elements, which are poorly organized due to the presence of phosphoserine. It represents about 11% of yolk proteins and is hydrophilic.

Application

Phosvitin from egg yolk has been used:
  • as a control protein for immobilization on-chip for deep purple protein staining methods for monitoring post-translation modifications
  • as a substrate for casein kinase Iε in in vitro casein kinase assay
  • as a standard in gel filtration chromatography (GFC) and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) for quantification of phosvitin from egg extracts
  • for immobilization onto Layer-by-Layer (LbL) for Fourier transform infrared spectroscopy-attenuated total reflectance (FTIR-ATR) studies

Biochem/physiol Actions

Phosvitin fed to rats by adding egg yolk to their food resulted in decreased absorption of magnesium, iron and calcium but not of phosphorus.
Phosvitin from egg yolk is a calcium chelator and binds to iron present in the yolk. The metal-binding functionality is exploited in mineral-binding bioactive peptide production. It is a nutraceutical and has potential for phosphopeptides production.

Other Notes

A phosophoprotein containing 8-10% phosphorus. Molar N/P ratio approx. 2.7.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Chen Sun et al.
Developmental and comparative immunology, 40(1), 28-34 (2013-01-12)
Lymphocystis disease virus (LCDV), a virus of Iridoviridae, can infect numerous teleost species, causing serious losses of aquaculture industry, and thus effective ways of prophylaxis and treatment are demanded. Previous studies have shown that phosvitin (Pv) is an antimicrobial agent
P L Lowrey et al.
Science (New York, N.Y.), 288(5465), 483-492 (2000-04-25)
The tau mutation is a semidominant autosomal allele that dramatically shortens period length of circadian rhythms in Syrian hamsters. We report the molecular identification of the tau locus using genetically directed representational difference analysis to define a region of conserved
Bo Lei et al.
Journal of chromatography. A, 1223, 41-46 (2011-12-27)
The objective of this study was to develop a simple method of phosvitin purification from hen egg yolk without using organic solvents. Egg yolk was diluted with equal volume of water and stirred for one hour at room temperature, followed
Kodai Yamane et al.
Fish physiology and biochemistry, 39(2), 373-390 (2012-08-25)
Elasmobranchs (sharks and rays) exhibit unique reproductive characteristics and, in contrast to the situation in teleosts, very little is known about the identity, structure and physical characteristics of their egg yolk proteins. The aims of this study were to (1)
Denise Young et al.
Journal of agricultural and food chemistry, 59(17), 9207-9218 (2011-08-02)
Oxidative stress is involved in the initiation and propagation of chronic intestinal pathologies. Bioactive peptides such as egg yolk-derived phosvitin phosphopeptides (PPP3) have been previously shown to reduce in vitro oxidative stress by up-regulating glutathione synthesis and antioxidant enzyme activities.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service