N2250

Naphthol AS-BI phosphate disodium salt hydrate

fluorogenic, ≥96.0% (HPLC), powder

• Synonym(s): 2-Naphthalenecarboxamide, 7-bromo-N-(2-methoxyphenyl)-3-(phosphonooxy)-, disodium salt hydrate
• Empirical Formula (Hill Notation): C₁₈H₁₃BrNNa₂O₆P · xH₂O
• CAS Number: 207569-04-8
• Molecular Weight: 496.16 (anhydrous basis)
• UNSPSC Code: 12352204
• PubChem Substance ID: 24897558
• NACRES: NA.83

Properties

• Product Name: Naphthol AS-BI phosphate disodium salt hydrate, ≥96.0% (HPLC)
• Quality Segment: 200
• assay: ≥96.0% (HPLC)
• form: powder
• solubility: water: 50 mg/mL, clear, colorless to yellow
• storage temp.: −20°C
• SMILES string: O.[Na+].[Na+].COc1ccccc1NC(=O)c2cc3cc(Br)ccc3cc2OP([O-])([O-])=O
• InChI: 1S/C18H15BrNO6P.2Na.H2O/c1-25-16-5-3-2-4-15(16)20-18(21)14-9-12-8-13(19)7-6-11(12)10-17(14)26-27(22,23)24;;;/h2-10H,1H3,(H,20,21)(H2,22,23,24);;;1H2/q;2*+1;/p-2
• InChI key: WNPKMQCBZKBNOS-UHFFFAOYSA-L

Description

General description

Substrate for the histochemical demonstration of acid and alkaline phosphatase.

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) is a substituted naphthol.

Application

Naphthol AS-BI phosphate disodium salt hydrate is suitable to use:

• in tartrate-resistant acid phosphatase (TRAP) staining to detect osteoclast activity
• as a component in Tris-HCl in staining cells to measure alkaline phosphatase (ALP) activity
• to perform alkaline phosphatase reaction

Biochem/physiol Actions

Naphthol AS-BI (7-Bromo-3-hydroxy-2-naphtho-o-anisidine) can be used as a substrate to determine alkaline and acid phosphatase. It is also used to determine the activity of acid phosphatase in single cells.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)