M7257
α-Mannosidase from Canavalia ensiformis (Jack bean)
ammonium sulfate suspension, ≥15 units/mg protein (biuret)
Synonym(s):
α-D-Mannoside mannohydrolase
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About This Item
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biological source
Canavalia ensiformis
form
ammonium sulfate suspension
specific activity
≥15 units/mg protein (biuret)
foreign activity
β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%
storage temp.
2-8°C
SMILES string
[Zn+2]
InChI
1S/Zn/q+2
InChI key
PTFCDOFLOPIGGS-UHFFFAOYSA-N
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General description
α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.
α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.
Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.
Application
α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.
α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:
- for the digestion of neutral glycans.
- to remove O-glycans from recombinant β-glucosidase
- for the inhibition of renal fibroblasts proliferation
Liberates mannose from a variety of synthetic and natural α-mannosides.
Biochem/physiol Actions
α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.
α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.
α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.
Unit Definition
One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.
Physical form
Suspension in 3.0 M (NH4)2SO4 and 0.1 mM zinc acetate, pH 7.5
inhibitor
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Description
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substrate
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Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Resp. Sens. 1
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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W Einhoff et al.
Biological chemistry Hoppe-Seyler, 369(3), 165-169 (1988-03-01)
The alpha-mannosidase from Canavalia ensiformis was characterized with respect to molecular mass, glycoprotein nature, amino-acid composition, enzymic properties and action on animal cells. The enzyme is composed of two pairs of subunits (molecular mass 44 and 66 kDa) which form
Engineering a highly active thermophilic beta-glucosidase to enhance its pH stability and saccharification performance
Xia W, et al.
Biotechnology for Biofuels, 9(1), 147-147 (2016)
Jack bean alpha-mannosidase: Amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool.
Kumar G, et al.
Glycobiology, 24(3) (2014)
Reto Buser et al.
The Journal of biological chemistry, 285(14), 10715-10723 (2010-01-12)
Coprinopsis cinerea is a model organism for fruiting body development in homobasidiomycetes. Here, we focused on N-linked oligosaccharides (NLO) of cell wall proteins in the hyphae of two developmental stages, vegetative mycelium and fruiting body. High mannose-type glycans were the
Collectin-11 promotes the development of renal tubulointerstitial fibrosis
Wu W, et al.
Journal of the American Society of Nephrology, 29, 168-181 (2018)
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