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Pyrophosphatase, Inorganic from Escherichia coli

Name: Pyrophosphatase, Inorganic from <I>Escherichia coli</I>
Brand: SIGMA

recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein

Synonym(s):

Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase

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About This Item

CAS Number:

9024-82-2

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

232-784-7

MDL number:

MFCD00131379

EC Number:

3.6.1.1 (BRENDA, IUBMB)

Specific activity:

≥800 units/mg protein

Assay:

≥90%

Recombinant:

expressed in E. coli

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Properties

recombinant

expressed in E. coli

Quality Segment

200

assay

≥90%

form

lyophilized powder

specific activity

≥800 units/mg protein

mol wt

hexamer subunit mol wt 20 kDa

storage temp.

−20°C

Description

General description

Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.[1]

Application

Pyrophosphatase, Inorganic from Escherichia coli has been used as a component of transcription buffer.

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H₂O → 2P_i.
It plays an important role in protein, RNA, and DNA synthesis.

Pyrophosphatase, inorganic from Escherichia coli has been used in assay for conjugation of ubiquitin and ubiquitin-like proteins.[2] It has been used for one-pot three-enzyme system for synthesis of Lewis x and sialyl Lewis x antigens.[3]

Biochem/physiol Actions

Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium.

Physical form

Lyophilized powder in Tris-buffered salts containing protease inhibitors

Other Notes

A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg²⁺ dependent. It is a relatively thermostable protein.

One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.

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This Item	P5931	I1643	F3174
Sigma-Aldrich I5907 Pyrophosphatase, Inorganic from Escherichia coli	Sigma-Aldrich P5931 Phosphatase, Alkaline from Escherichia coli	Sigma-Aldrich I1643 Pyrophosphatase, Inorganic from baker′s yeast (S. cerevisiae)	Sigma-Aldrich F3174 Fpg Protein from Escherichia coli
specific activity ≥800 units/mg protein	specific activity 30-60 units/mg protein (in glycine buffer)	specific activity ≥500 units/mg protein (E1%/280)	specific activity >20,000 units/mg protein
assay ≥90%	assay -	assay -	assay ≥90% (SDS-PAGE)
form lyophilized powder	form lyophilized powder	form powder	form buffered aqueous glycerol solution
recombinant expressed in E. coli	recombinant -	recombinant -	recombinant expressed in E. coli
mol wt hexamer subunit mol wt 20 kDa	mol wt -	mol wt 71 kDa	mol wt 30.2 kDa (269 amino acids, predicted from the nucleotide sequence)
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C

pictograms

GHS07

signalword

Warning

hcodes

H315,H319,H335

pcodes

P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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