GE28-9031-34

Protein G High Performance Spintrap^™

Cytiva 28-9031-34, pack of 16 columns

• Synonym(s): Protein G Column
• UNSPSC Code: 41106500
• NACRES: NA.25

Properties

• ligand: recombinant protein G lacking albumin-binding region
• feature: wetted part: no
• packaging: pack of 16 columns
• manufacturer/tradename: Cytiva 28-9031-34
• storage condition: (20% Ehtanol); (20% Ethanol)
• matrix: highly cross-linked 6% agarose
• avg. part. size: 34 μm
• cleaning: 2-9
• working range: 3-9
• capacity: >1 mg binding capacity (lgG/column)(The binding capacity has been determined with human polyclonal IgG.); >1 mg binding capacity(human IgG/column)
• storage temp.: 2-8°C

Description

General description

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

pH below 3 is sometimes required to elute strongly bound IgG species. However, protein ligands may hydrolyze at very low pH.

protein G HP SpinTrap^™ is a prepacked, single-use spin column containing protein G Sepharose^™ High Performance.

Application

Protein G Sepharose^™ HP has high affinity for the Fc region of IgG from a variety of species. The two options in the Protein enrichment protocol allow the attached antigen to be eluted separately (cross-link protocol) or together with the antibody (classic protocol).


There is also a protocol for efficient, small-scale purification of monoclonal and polyclonal antibodies from serum and cell culture supernatants in less than 20 min. No pretreatment of the sample is required.

Features and Benefits

• For simple, small-scale purification of antibodies or enrichment of specific Proteins in prepacked spin columns that are ready to use.
• Classic and cross-link protocols provide flexibility.
• Elution conditions formatted for both electrophoresis and LC-MS analysis workflows.
• Fast binding kinetics and high capacity provides high yield.
• Prepacked with Protein G Sepharose^™ High Performance for coupling of antibodies of IgG subclasses.
• Easy scale-up with HiTrap^® Protein G HP prepacked columns.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

HiTrap is a registered trademark of Cytiva

Sepharose is a trademark of Cytiva

SpinTrap is a trademark of Cytiva

Safety Information

• Pictograms: GHS02
• Signal Word: Warning
• Hazard Statements: H226
• Precautionary Statements: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class Code: 3 - Flammable liquids