GE17-5281-01
GSTrap™ High Performance
Cytiva 17-5281-01, pack of 5 × 1 mL
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About This Item
UNSPSC Code:
23151816
NACRES:
NA.56
Recommended Products
ligand
glutathione
packaging
pack of 5 × 1 mL
manufacturer/tradename
Cytiva 17-5281-01
parameter
42 psi (H2O at room temperature.)
bed size
7 mm × 25 mm
bed volume
1 mL
column I.D.
7 mm
matrix
highly cross-linked 6% agarose
avg. part. size
34 μm
cleaning
3-12
working range
3-12
capacity
>7 mg/mL binding capacity(H2O at room temperature.)
Related Categories
General description
GSTrap™ HP columns are prepacked with Glutathione Sepharose™ High Performance medium and provide high-resolution, one-step purifications of glutathione S-Transferase (GST) tagged proteins.
Application
GSTrap™ HP 1-ml and 5-ml columns are prepacked with Glutathione Sepharose™ High Performance medium, which provides high-resolution purifications, thus a more concentrated sample. GST-tagged Proteins are purified from pretreated bacterial lysates by affinity chromatography using immobilized glutathione, such as Glutathione Sepharose™ High Performance. GST-tagged Proteins are bound to the affinity medium, and impurities are removed by washing with binding buffer.
GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.
GST-tagged Proteins are eluted under mild, nondenaturing conditions using reduced glutathione. The purification process preserves Protein antigenicity and function. If desired, cleavage of the Protein from GST can be achieved using a site-specific protease whose recognition sequence is located immediately upstream from the multiple cloning site on the pGEX plasmids. GST-tagged Proteins can be detected using colorimetric or immunological methods.
Features and Benefits
- High resolution purification of GST-tagged Proteins.
- Mild elution conditions preserving Protein antigenicity and function.
- Fast, one-step purification.
- Easy to use with syringe, pump or a chromatography system.
Storage and Stability
Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)
Analysis Note
To view the Certificate of Analysis for this product, please visit www.cytiva.com.
Other Notes
The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.
Legal Information
GSTrap is a trademark of Cytiva
Sepharose is a trademark of Cytiva
Signal Word
Warning
Hazard Statements
Precautionary Statements
Storage Class Code
3 - Flammable liquids
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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