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G4166

Sigma-Aldrich

Glucose Isomerase from Streptomyces murinus

≥350 U/g

Synonym(s):

Sweetzyme® IT Extra, D-xylose ketol-isomerase

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About This Item

Enzyme Commission number:
5.3.1.5 (BRENDA, IUBMB)
MDL number:
MFCD00675436
UNSPSC Code:
12352204
NACRES:
NA.54
Pricing and availability is not currently available.

biological source

Streptomyces sp. (S. murinus)

Quality Level

200

form

powder

specific activity

≥350 U/g

storage temp.

2-8°C

General description

Immobilized glucose isomerase produced from a selected strain of Streptomyces murinus.

Application

Glucose Isomerase from Streptomyces murinus has been used to isomerize xylose to xylulose during the production of dihydrogen from Xylose.[1][2] It has also been used in the synthetic enzymatic pathway for dihydrogen production from sucrose, to catalyze isomerization of glucose to fructose.[2]
Immobilized glucose isomerase produced from Streptomyces murinus was used for the isomerization of xylose.[3] Glucose isomerase is used in the food industry to produce high-fructose corn syrup[4].

Biochem/physiol Actions

Few anaerobic bacteria, fungi and plants express an intracellular metalloenzyme called D-xylose isomerase (XI). Most bacteria use the enzyme D-xylose isomerase to transform D-xylose to D-xylulose. D-Xylose isomerase (XI) converts the aldo-sugars xylose and glucose to their keto analogs xylulose and fructose.[5]
Glucose isomerase has wide variety of industrial applications such as producing high-fructose corn syrup (HFCS) and production of ethanol from hemicelluloses. In addition, it also facilitates the study of structure-function relationships by advanced biochemical and genetic engineering techniques.[6]

Physical properties

0.33 g yields an approximate bed volume of 1ml

Unit Definition

one unit converts glucose to fructose at an initial rate of 1 μmole per min at standard analytical conditions

Legal Information

A product of Novozyme Corp.
Sweetzyme is a registered trademark of Novozymes Corp.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
0000296837
0000295495
SLCP9134
SLCL0514
SLCB7879

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Leonardo de Figueiredo Vilela et al.
Bioresource technology, 128, 792-796 (2012-11-29)
This study presents results regarding the successful cloning of the bacterial xylose isomerase gene (xylA) of Burkholderia cenocepacia and its functional expression in Saccharomyces cerevisiae. The recombinant yeast showed to be competent to efficiently produce ethanol from both glucose and
Kripa Rao et al.
Applied biochemistry and biotechnology, 146(1-3), 101-117 (2008-04-19)
Of the sugars recovered from lignocellulose, D-glucose can be readily converted into ethanol by baker's or brewer's yeast (Saccharomyces cerevisiae). However, xylose that is obtained by the hydrolysis of the hemicellulosic portion is not fermentable by the same species of
Hiroki Kitayama et al.
Biochimica et biophysica acta, 1834(12), 2640-2646 (2013-10-08)
Protein crystals form in supersaturated solutions via a nucleation and growth mechanism. The amyloid fibrils of denatured proteins also form via a nucleation and growth mechanism. This similarity suggests that, although protein crystals and amyloid fibrils are distinct in their
Molecular and industrial aspects of glucose isomerase.
Bhosale SH,et al.
Microbiology and Molecular Biology Reviews, 60(2), 280-300 (1996)
Dominik Oberthuer et al.
PloS one, 7(6), e33545-e33545 (2012-06-08)
In this paper, we demonstrate the feasibility of using in situ Dynamic Light Scattering (DLS) to monitor counter-diffusion crystallization experiments in capillaries. Firstly, we have validated the quality of the DLS signal in thin capillaries, which is comparable to that
View All Related Papers

Questions

1–2 of 2 Questions  

  1. Is G4166 an immobilized enzyme? If it is, what support is it immobilized on?

    1 answer

    1. This is a Novozyme product, Sweetzyme® IT Extra, which is an immobilized enzyme. It is glutaraldehyde crosslinked on polyethyleneimine, and intended to be used in a continuous fixed bed process (converting high dextrose syrup to high fructose syrup).

      Helpful?

  2. グルコースイソメラーゼ Streptomyces murinus由来(Cat.No G4166) が可溶化できません。どのように溶かすのでしょうか？またどのような溶液だと可能でしょうか？

    1 answer

    1. This is a product of Novozymes, available commercially as Sweetzyme. This is an immobilized enzyme used in a fixed bed process to convert dextrose syrup to fructose syrup. This material is in granular form with a particle size range of 0.3-1.0 mm. This material is not intended to be solubilized.

      Helpful?

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