F7182

Fibroblast Activation Protein α human

recombinant, expressed in Sf21 cells, ≥95% (SDS-PAGE)

• Synonym(s): FAP, Seprase
• NACRES: NA.54
• UNSPSC Code: 12352204
• Specific activity: ≥1800 units/μg protein
• Assay: ≥95% (SDS-PAGE)
• Recombinant: expressed in Sf21 cells

Properties

• recombinant: expressed in Sf21 cells
• Quality Segment: 200
• assay: ≥95% (SDS-PAGE)
• form: solution
• specific activity: ≥1800 units/μg protein
• impurities: ≤1.0 EU/μg endotoxin
• shipped in: dry ice
• storage temp.: −70°C

Description

General description

Fibroblast activation protein alpha (FAPa) is a type II transmembrane serine protease. It is present as a homodimeric integral protein with dipeptidyl peptidase IV like fold. It has an α/β-hydrolase domain and an eight-bladed β-propeller domain.[1] It is not expressed in normal tissue. It is only expressed by activated fibroblast in response to pathologic situations. It is expressed in several pathogenic sites including cancer, fibrosis, arthritis, wounding, or inflammation.[2]

Application

FAPa may be used to study the role in coordinating the stromal response to cancer, in angiogenesis, and modulation of the antitumor immune response.[2]

Biochem/physiol Actions

FAP is a Type II transmembrane serine protease which has substrate specificity for prolyl peptides bonds (Pro-Xaa). This endopeptidase cleaves gelatin and is believed to play a role in tissue remodeling, tumor invasion, and wound repair.

FAPα has in vitro dipeptidyl peptidase activity and collagenolytic activity. It cleaves N-terminal dipeptides from polypeptides and can degrade gelatin and type I collagen. It has also been reported that FAPa has a tumor suppressor activity.[1]

Physical form

Supplied as a solution in 25 mM Tris, 0.25 M NaCl and 20% (v/v) glycerol , pH 8.0

Other Notes

One unit will convert 1 picomole of benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (ZP-AMC) to Z-gly-Pro and 7-amino-4-methylcoumarin (AMC) per minute at pH 7.5 at 25°C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable