The stability of this Fibronectin solution upon further dilution has not been evaluated. At the prepared 1 mg/mL concentration, the solution is stable for at least 1 year from the date of shipment, when stored at 2-8°C. Please see the following links to review additional information regarding usage and handling of Fibronectin:
Coating protocol
https://www.sigmaaldrich.com/US/en/technical-documents/protocol/cell-culture-and-cell-culture-analysis/cell-growth-and-maintenance/fibronectin-product-protocols
General information
https://www.sigmaaldrich.com/US/en/technical-documents/technical-article/cell-culture-and-cell-culture-analysis/mammalian-cell-culture/fibronectin
F1141
Bovine Fibronectin
from bovine plasma, liquid, suitable for cell culture
Synonym(s):
Fibronectin
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| Pack Size | SKU | Availability | Price |
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About This Item
Biological source:
bovine plasma
Form:
solution
Technique(s):
cell culture | mammalian: suitable
Pricing and availability is not currently available.
Product Name
Fibronectin bovine plasma, solution, sterile-filtered, BioReagent, suitable for cell culture
biological source
bovine plasma
Quality Level
sterility
sterile-filtered
product line
BioReagent
form
solution
packaging
pkg of 1 mg, pkg of 2 mg, pkg of 5 mg
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑5 μg/cm2
UniProt accession no.
binding specificity
Peptide Source: Collagen
, Peptide Source: Fibrinogen
shipped in
wet ice
storage temp.
2-8°C
Gene Information
bovine ... FN1(280794)
General description
Fibronectin is a ubiquitous protein of the extracellular matrix. It is a mosaic protein, that is made up of three types of repeating modular consensus amino acid sequences. Fibronectins belongs to a class of high-molecular-weight glycoproteins. Cellular fibronectin is expressed by fibroblasts and plasma fibronectin is usually liberated by hepatocytes.
Application
Fibronectin (FN) bovine plasma has been used in:
- fibronectin and adsorption analysis (ELISA (enzyme-linked immunosorbent assay))
- transwell migration
- fibronectin assay
Biochem/physiol Actions
Fibronectins participate in cell adhesion, cytoskeletal organization, oncogenic transformation, cell migration, phagocytosis, hemostasis, and embryonic differentiation.
Analysis Note
Protein homogeneity is evaluated by immunoelectrophoresis.
Other Notes
Product F1141, Fibronectin from bovine plasma is a solution in 0.05 M TBS pH 7.5.
1 of 1
This Item | |||
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| sterility sterile-filtered | sterility sterile | sterility sterile | sterility sterile |
| biological source bovine plasma | biological source bovine plasma | biological source human plasma | biological source human plasma |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| form solution | form powder | form liquid | form lyophilized powder |
| shipped in wet ice | shipped in dry ice | shipped in wet ice | shipped in dry ice |
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signalword
Danger
hcodes
Hazard Classifications
Resp. Sens. 1 - Skin Sens. 1
Storage Class
12 - Non Combustible Liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves
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CCL2/CCR2 chemokine signaling coordinates survival and motility of breast cancer cells through Smad3 and p42/44MAPK dependent mechanisms
Fang W B, et al.
The Journal of biological chemistry, jbc-M112 (2012)
FIBRONECTIN AUTOANTIBODIES
Autoantibodies, 221-251 (2007)
Enhanced fibronectin adsorption on carbon nanotube/poly (carbonate) urethane: independent role of surface nano-roughness and associated surface energy
Khang D, et al.
Biomaterials, 28(32), 4756-4768 (2007)
Global Trade Item Number
| SKU | GTIN |
|---|---|
| F1141-5MG | 04061835514144 |
| F1141-1MG | 04061835568444 |
| F1141-2MG | 04061833611678 |
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How long can I store the diluted solution (20µg/ml) in PBS?
1 answer-
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Can I freeze the aliquoted Fibronectin?
1 answer-
The recommended storage temperature for this product is 2-8°C. While freezer storage is not required, storing this solution in frozen aliquots at -20°C will not impact the integrity of the protein. Avoid repeated freeze/thaw cycles. The long term stability of this material when stored frozen has not been evaluated and may not result in extended shelf life.
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Hi, I bought a vial with 5mg of Fibronectin (F1141), but so far, I have not been able to find the concentration of this solution. Can you please help me with this info? Thanks a lot in advance!
1 answer-
The concentration of this product is 1 mg/mL. This information is reported in the product Certificate of Analysis. Please see the link below to review a sample or lot-specific Certificate:
https://www.sigmaaldrich.com/product/sigma/f1141#product-documentationHelpful?
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How long can I store fibronectin coated plates, etc?
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Fibronectin coated cultureware can be stored for 2-4 weeks at 2-8 °C in a closed sterile container, or in sterile sealable bags.
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What is the procedure for coating culture surfaces with fibronectin?
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To coat culture surfaces: 1. Dilute fibronectin in sterile balanced salt solution and coat the culture surface (1-5 μg/cm2) with a minimal volume. 2. Allow to air dry for at least 45 minutes at room temperature. Excess fibronectin may be removed by aspiration, but this is not necessary.
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What is the Department of Transportation shipping information for this product?
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Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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How long can I store the fibronectin solution?
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The product is good for at least a year at 2-8 °C if kept sterile.
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How do I choose which fibronectin product to use to coat culture surfaces for cell growth?
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The species source for the fibronectin used when coating culture surfaces is not important. It is the presence of the RGD binding site that is present in all fibronectins that promotes binding.
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