This product is provided as a slurry containing 10 mg of enzyme in saturated ammonium sulfate precipitate. It is supplied at 100 units, with a concentration of 0.13 units per 1 mg of protein at 10 mg per ml.
E6412
Cellobiohydrolase I from Hypocrea jecorina
100 U/mg, recombinant, expressed in corn
Synonym(s):
Cel7A, Cellobiosidase, Cellulase
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recombinant
expressed in corn
Quality Level
form
liquid
specific activity
100 U/mg
greener alternative product characteristics
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sustainability
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greener alternative category
shipped in
dry ice
storage temp.
−20°C
General description
Cellubiohydrolase I is an enzyme present in many fungi, but particularly wood rot fungi. It is a monomer of 53 kDa with a catalytic domain and a cellulose binding domain. The reaction adds water to the glucose bonds in cellulose (non-reducing ends of the chain), yielding cellobiose.
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Application
Cellobiohydrolase I can be used in combination with endocellulases and b-glucosidase to produce glucose from cellulose.
Biochem/physiol Actions
Cellobiohydrolase (CBH) is a cellulase which degrades cellulose by hydrolysing the 1,4-β-D-glycosidic bonds. CBH is an exocellulase which cleaves two to four units from the ends of cellulose. CBH I cleaves progressively from the reducing end. CBH I is commonly used in detergents for cleaning textiles. Its ezymatic activity ranges from 37° C to 50° C, with its optimal temperature being approximately 45° C. The optimum pH for the enzyme is 5-6.
Physical form
Provided as an ammonium sulfate precipitate with the source as recombinant maize.
Other Notes
Unit Definition: A unit will turn over 1 nmole of methyl-umbelliferyl beta-D cellobioside per min at pH 5 at 50° C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity 100 U/mg | specific activity - | specific activity 0.3-3.0 unit/mg solid (using a β-galactose dehydrogenase system and locust bean gum as substrate) | specific activity ≥700 units/g |
| form liquid | form powder | form powder | form aqueous solution |
| recombinant expressed in corn | recombinant - | recombinant - | recombinant - |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| shipped in dry ice | shipped in wet ice | shipped in - | shipped in - |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Larissa C Textor et al.
The FEBS journal, 280(1), 56-69 (2012-11-02)
Aiming to contribute toward the characterization of new, biotechnologically relevant cellulolytic enzymes, we report here the first crystal structure of the catalytic core domain of Cel7A (cellobiohydrolase I) from the filamentous fungus Trichoderma harzianum IOC 3844. Our structural studies and
Svein J Horn et al.
Methods in enzymology, 510, 69-95 (2012-05-23)
Natural cellulolytic enzyme systems as well as leading commercial cellulase cocktails are dominated by enzymes that degrade cellulose chains in a processive manner. Despite the abundance of processivity among natural cellulases, the molecular basis as well as the biotechnological implications
Kiyohiko Igarashi et al.
Methods in enzymology, 510, 169-182 (2012-05-23)
Cellulases hydrolyze β-1,4-glucosidic linkages of insoluble cellulose at the solid/liquid interface, generating soluble cellooligosaccharides. We describe here our method for real-time observation of the behavior of cellulase molecules on the substrate, using high-speed atomic force microscopy (HS-AFM). When glycoside hydrolase
Global Trade Item Number
| SKU | GTIN |
|---|---|
| E6412-100UN | 04061833604526 |
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