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Endothelial cell growth supplement from bovine pituitary

Name: Endothelial cell growth supplement from bovine pituitary
Brand: SIGMA

Bioreagent, powder, suitable for cell culture

Synonym(s):

ECGS, Endothelial Mitogen

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About This Item

UNSPSC Code:

12352202

NACRES:

NA.77

Biological source:

bovine pituitary glands

Form:

powder

Impurities:

≤20 EU/vial (LAL test)

Pricing and availability is not currently available.

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Properties

Product Name

Endothelial cell growth supplement from bovine pituitary, ECGS, BioReagent, suitable for cell culture

biological source

bovine pituitary glands

Quality Level

200

product line

BioReagent

form

powder

potency

2-300 μg/mL ED₅₀/EC₅₀

quality

endotoxin tested

packaging

pkg of 15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

brown to tan

solubility

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

Description

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:

as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
as a supplement in medium 199 for endothelial cell culture
as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

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This Item	E2759	P5208	E1388
Sigma-Aldrich E0760 Endothelial cell growth supplement from bovine pituitary	Sigma-Aldrich E2759 Endothelial cell growth supplement from bovine neural tissue	Sigma-Aldrich P5208 Platelet-Derived Endothelial Cell Growth Factor human	Sigma-Aldrich E1388 β-Endothelial Cell Growth Factor human
technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable	technique(s) cell culture \| mammalian: suitable
biological source bovine pituitary glands	biological source bovine neuroblastoma	biological source human	biological source human
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200
form powder	form powder	form lyophilized powder	form lyophilized powder
impurities ≤20 EU/vial (LAL test)	impurities ≤20 EU/vial (LAL test)	impurities <1 EU/mg	impurities ≤1.0 EU/μg protein Endotoxin level (LAL test)
storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles	storage condition avoid repeated freeze/thaw cycles (Do not store in a frost-free freezer.)

pictograms

GHS08

signalword

Warning

hcodes

H361fd

pcodes

P201 - P202 - P280 - P308 + P313 - P405 - P501

Hazard Classifications

Repr. 2

Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Global Trade Item Number

SKU	GTIN
E0760-15MG	04061835514120

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