D8783
7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt
10 mM in H2O
Synonym(s):
−N7-dGTP, 7-Deaza-dGTP
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About This Item
Empirical Formula (Hill Notation):
C11H17N4O13P3
CAS Number:
Molecular Weight:
506.19
NACRES:
NA.52
PubChem Substance ID:
UNSPSC Code:
41106305
MDL number:
Assay:
≥95% (HPLC)
Biological source:
Porcine muscle, rabbit muscle
Form:
liquid
Storage temp.:
−20°C
Quality Level
biological source
Porcine muscle, rabbit muscle
assay
≥95% (HPLC)
form
liquid
feature
PCR Additives
concentration
10 mM in H2O
technique(s)
PCR: suitable
color
colorless
shipped in
dry ice
storage temp.
−20°C
SMILES string
NC1=NC(=O)c2ccn(C3CC(O)C(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)O3)c2N1
InChI
1S/C11H17N4O13P3/c12-11-13-9-5(10(17)14-11)1-2-15(9)8-3-6(16)7(26-8)4-25-30(21,22)28-31(23,24)27-29(18,19)20/h1-2,6-8,16H,3-4H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,12,13,14,17)
InChI key
DLLXAZJTLIUPAI-UHFFFAOYSA-N
Application
7-Deaza-2′-deoxyguanosine 5′-triphosphate lithium salt has been used as a lysis agent to evaluate its efficiency on direct cell lysis, RNA stability, and compatibility with downstream reverse transcription quantitative real-time PCR during the analyzes of samples with small numbers of cells.
Biochem/physiol Actions
7-Deaza-2′-deoxyguanosine 5′-triphosphate (7-deaza-dGTP) competes with natural substrate dGTP and blocks the telomerase activity. This nucleotide once incorporated into the telomere alters the secondary structure of the telomere making it difficult for telomerase to identify it for further telomere synthesis. This dGTP analog pairs weakly with conventional bases to minimize DNA secondary structures, while being a good substrate for DNA polymerases. 7-deaza-dGTP in combination with dimethyl sulfoxide (DMSO) and betaine enhances the polymerase chain reaction (PCR) amplification of GC-rich DNA sequences.
Storage Class
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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Russell J Diefenbach et al.
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Detection of melanoma-associated mutations using circulating tumor DNA (ctDNA) from plasma is a potential alternative to using genomic DNA from invasive tissue biopsies. In this study, we developed a custom melanoma next-generation sequencing (NGS) panel which includes 123 amplicons in
Michael Field et al.
International journal of molecular sciences, 20(16) (2019-08-14)
Although fragile X syndrome (FXS) is caused by a hypermethylated full mutation (FM) expansion with ≥200 cytosine-guanine-guanine (CGG) repeats, and a decrease in FMR1 mRNA and its protein (FMRP), incomplete silencing has been associated with more severe autism features in
Paulina H Wanrooij et al.
Nucleic acids research, 40(20), 10334-10344 (2012-09-12)
In human mitochondria the transcription machinery generates the RNA primers needed for initiation of DNA replication. A critical feature of the leading-strand origin of mitochondrial DNA replication is a CG-rich element denoted conserved sequence block II (CSB II). During transcription
Global Trade Item Number
| SKU | GTIN |
|---|---|
| D8783-.5UMO | 04061833589915 |