C0918
Fluorimeter cuvettes
Polystyrene, without stopper
Synonym(s):
cuvette, plastic cuvette, plastic cuvettes
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About This Item
UNSPSC Code:
12000000
NACRES:
SB.32
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parameter
340-750 nm spectral range
technique(s)
photometry: suitable
L × W × H
12.7 mm × 12.7 mm × 44.7 mm
pathlength
10 mm
volume
≥3 mL
detection method
fluorometric
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General description
See transmission curve labelled D
These cuvettes have four optically clear sides.
Note: polystyrene and polymethacrylate cuvettes are incompatible with some organic solvents.
Note: polystyrene and polymethacrylate cuvettes are incompatible with some organic solvents.
Application
Fluorimeter cuvette may be used :
- to perform a fluorimetric assay of the hepatic microsomal O-dealkylation reaction, using ethoxyresorufin (7-ethoxyphenoxazone) as a model substrate for the assay.
- in the cationic liposome-mediated gene transfer method, done in order to deduce the role of helper lipids.
- in the fluorescence experiment modified in the Silber-Porter method, for estimation of corticosterone in rat plasma.
- in fluorimeteric assay for membrane potential to determine membrane depolarization in study done to elucidate the role of membrane potential in the mechanism of action of daptomycin.
Compatibility
Not compatible with some organic solvents.
Certificates of Analysis (COA)
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The estimation of small amounts of corticosterone in rat plasma.
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Jared A Silverman et al.
Antimicrobial agents and chemotherapy, 47(8), 2538-2544 (2003-07-25)
The objective of this study was to further elucidate the role of membrane potential in the mechanism of action of daptomycin, a novel lipopeptide antibiotic. Membrane depolarization was measured by both fluorimetric and flow cytometric assays. Adding daptomycin (5 micro
Ethoxyresorufin: direct fluorimetric assay of a microsomal O-dealkylation which is preferentially inducible by 3-methylcholanthrene.
M D Burke et al.
Drug metabolism and disposition: the biological fate of chemicals, 2(6), 583-588 (1974-11-01)
S W Hui et al.
Biophysical journal, 71(2), 590-599 (1996-08-01)
In the procedure for cationic liposome-mediated transfection, the cationic lipid is usually mixed with a "helper lipid" to increase its transfection potency. The importance of helper lipids, including dioleoylphosphatidylcholine (DOPC) and phosphatidylethanolamine (dioleoyl PE), DO was examined. Freeze-fracture electron microscopy
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