A6680
N-Acetylneuraminic Acid Aldolase from microorganisms
lyophilized powder, ≥20 units/mg protein (biuret)
Synonym(s):
N-Acetylneuraminate Pyruvate Lyase, N-Acetylneuraminic Acid Lyase, NANA Aldolase, Sialic Aldolase
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About This Item
CAS Number:
NACRES:
NA.32
UNSPSC Code:
12352204
Beilstein/REAXYS Number:
2697172
MDL number:
Specific activity:
≥20 units/mg protein (biuret)
Pricing and availability is not currently available.
form
lyophilized powder
Quality Segment
specific activity
≥20 units/mg protein (biuret)
mol wt
~98 kDa
storage temp.
−20°C
General description
Isoelectric point: 4.6 ± 0.1
Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)
Michaelis constant: 2.5 x 10‾3M (N-Acetylneuraminic acid)
Structure: 3 subunits (approx. 35,000) per mol of enzyme
Inhibitors: p-Chloromercuribenzoate, sodium dodecyl sulfact, Hg++, Ag+
Optimum pH: 7.5– 8.0
Optimum temp: 70°C
pH Stability: pH 6.0–9.0 (10°C, 25hr)
Thermal stability: Below 65°C (pH 7.5, 30 min)
Application
This enzyme is useful for enzymatic determination of N-acetylneuraminic acid and sialic acid when
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
coupled with the related enzymes in clinical analysis.
For industrial use, this enzyme is useful for enzymatic synthesis of sialic acid.
Used in the Sialic Acid Quantification Kit, SIALIC-Q
Physical form
Lyophilized powder containing mannitol and EDTA
Other Notes
One unit will release 1.0 μmole of pyruvate from NANA per min at pH 7.7 at 37 °C.
1 of 1
This Item | |||
|---|---|---|---|
| specific activity ≥20 units/mg protein (biuret) | specific activity ≥8.0 units/mg protein | specific activity ≥3.0 units/mg protein | specific activity 10-20 units/mg protein |
| form lyophilized powder | form lyophilized powder | form lyophilized powder | form ammonium sulfate suspension |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. 2-8°C |
| mol wt ~98 kDa | mol wt - | mol wt 33.4 kDa | mol wt - |
| Quality Level 200 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
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Global Trade Item Number
| SKU | GTIN |
|---|---|
| A6680-25UN | 04061832852782 |