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83702

Sigma-Aldrich

Rhodamine 123

BioReagent, for fluorescence, ≥85% (HPLC)

Synonym(s):

2-(6-Amino-3-imino-3H-xanthen-9-yl)benzoic acid methyl ester

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About This Item

Linear Formula:
C21H17ClN2O3
CAS Number:
62669-70-9
Molecular Weight:
380.82
Beilstein:
6030951
EC Number:
263-687-8
MDL number:
MFCD00012664
UNSPSC Code:
12352116
PubChem Substance ID:
57652966
NACRES:
NA.32
Pricing and availability is not currently available.

grade

for fluorescence

Quality Level

100

product line

BioReagent

Assay

≥85% (HPLC)

form

powder

impurities

≤10% water

solubility

ethanol: 20 mg/mL, clear, red

suitability

in accordance for fluorescence

SMILES string

Cl[H].COC(=O)c1ccccc1C2=C3C=CC(=N)C=C3Oc4cc(N)ccc24

InChI

1S/C21H16N2O3.ClH/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20;/h2-11,22H,23H2,1H3;1H

InChI key

MYFATKRONKHHQL-UHFFFAOYSA-N

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General description

Rhodamine 123 is a fluorescent dye.[1]

Application

Rhodamine 123 has been used:
  • as a substrate for P-glycoprotein (P-gp) to read out functional transporter activity in fluorescent accumulation assays[2]
  • to label the cells for estimating the P-gp expression by flow cytometry[1]
  • in transport studies, was added to the apical to basolateral (AB-transport) or basolateral to apical (BA-transport)[3]
  • the detection of mitochondrial membrane potential (ΔΨm)[4]

Biochem/physiol Actions

Fluorescent dye most commonly used in flow cytometry as functional reporter for Pgp. Functional assays for MDR proteins are better prognostic indicators in cancer therapy than levels of MDR protein expression. Rh 123 can be used in multiparameter analyses without fluorescence interference in combination with common protein labeling dyes such as PE-Cy5 and AMCA (7-amino-4-methylcoumarin-3-acetic acid). Recent reports indicate Rh 123 may also be a substrate of MRP1.
Rhodamine 123 is usually used as a functional reporter for P-glycoprotein (P-gp) in flow cytometry.[1]

Other Notes

Supravital mitochondrial-specific dye[5]

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM6618
BCCL3983
BCCH9222
BCCG4466
BCCB7631

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Assessment of the chemotherapeutic potential of a new camptothecin derivative, ZBH-1205
Wu D, et al.
Archives of Biochemistry and Biophysics, 604, 74-85 (2016)
Differentiation and characterization of human pluripotent stem cell-derived brain microvascular endothelial cells
Stebbins MJ, et al.
Methods, 101, 93-102 (2016)
L Benel et al.
Basic and applied histochemistry, 33(2), 71-80 (1989-01-01)
The use of the supravital mitochondrial-specific dye Rhodamine 123 (Rh 123) in combination with flow cytometry permits the monitoring of the changes in the mitochondrial transmembrane potential, reflecting the overall mitochondrial activity of the living cell. While this probe appears
Evidence of apoptosis in Raillietina echinobothrida induced by methanolic extracts of three traditional medicinal plants of Northeast India
Giri BR, et al.
Experimental Parasitology, 134(4), 466-473 (2013)
X Ronot et al.
Biology of the cell, 57(1), 1-7 (1986-01-01)
Flow cytometry combines the advantages of microscopy and biochemical analysis in a single highly sensitive technique for a rapid examination of numerous individual living cells. It has become a potent and essential tool in the studies of the physiology of
View All Related Papers

Questions

1–5 of 5 Questions  

  1. Which is the storage conditions recommend for this product?

    1 answer

    1. This product may be stored at room temperature.

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  2. Why would one select Rhodamine 123 (Product Nos. 83702 and R8004) instead of other rhodamine compounds?

    1 answer

    1. Due to is comparatively low toxicity, Rhodamine 123 is the dye of choice for visualizing mitochondria or for cell viability measurements.

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  3. What is the solubility of Rhodamine 123, (Product Nos. 83702 and R8004)?

    1 answer

    1. Rhodamine 123 is soluble in ethanol at 20 mg/mL, and has been reported soluble in ether, various fats and lipids, and has limited solubility in water (no concentration given).

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  4. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  5. What are the excitation and emission wavelengths for Rhodamine 123 (Product Nos. 83702 and R8004)?

    1 answer

    1. Using ethanol as the solvent, the excitation maximum is  511 nm and the emission maximum is 534 nm.  The recommended excitation and emission wavelengths in PBS are 488 and 515-575 nm, respectively.

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