22091

Tryptic Soy Agar - Dehydrated Culture Media

NutriSelect^® Plus, powder, suitable for microbiology

• Synonym(s): CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soy Agar, CASO Agar, Soybean Casein digest Agar, TSA, Tryptone Soya Agar
• UNSPSC Code: 41171605
• NACRES: NA.74

Properties

• Agency: according to GB 4789.40-2016; according to ISO 11731:2017; according to ISO 22964:2017
• Quality Level: 200
• sterility: non-sterile
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• composition: agar, 15 g/L ; casein peptone (pancreatic), 15 g/L ; sodium chloride, 5 g/L ; soya peptone (papainic), 5 g/L
• packaging: bottle of 2.5 kg; bottle of 500 g
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | specific enzyme detection: suitable; microbiological culture: suitable
• color: light yellow
• final pH: 7.3±0.2 (25 °C)
• application(s): agriculture; bioburden testing; cleaning products; clinical testing; cosmetics; environmental; food and beverages; personal care; surface monitoring; veterinary; microbiology
• suitability: Enterococcus spp.; Haemophilus spp.; Pneumococcus spp.; nonselective for Bacillus spp.; nonselective for Bacteroides spp.; nonselective for Campylobacter spp.; nonselective for Candida spp.; nonselective for Cronobacter spp.; nonselective for Escherichia coli; nonselective for Neisseria spp.; nonselective for Staphylococcus spp.; nonselective for Streptococcus spp.; nonselective for bacteria (General Media); nonselective for coliforms

Description

General description

Tryptic Soy Agar is a general-purpose culture medium for the isolation and cultivation of fastidious and non-fastidious microorganisms or the maintenance of stock culture. It is a complex medium for the cultivation and isolation of a wide range of bacteria, yeasts, and molds. The formulation of the TSA medium contains a combination of casein and soy peptones supplying organic nitrogen, particularly amino acids, and longer-chained peptides. Sodium chloride maintains the osmotic equilibrium, and the natural sugars from soy peptone are the energy source.

Application

Tryptic Soy Agar is used for the pre-cultivation and enumeration (E. coli) by membrane filtration technique. It is suitable for the cultivation of both aerobic and anaerobic bacteria. Since it does not contain the X and V factors, it is suitable for the identification of Haemophilus sp. by adding X (Hemin) and V (DPN=NAD) factors strips.

Features and Benefits

NutriSelect^® Plus are powdered culture media offering

• Quality control with growth promotion test according to industry standards
• Quality assurance acc. to ISO 9001
• Cost-efficient powdered media

Preparation Note

Suspend 40 g of dehydrated media in 1 litre of purified filtered water. Sterilize at 121°C for 15 minutes. Cool to 45-50°C. Mix gently and dispense into sterile Petri dishes or sterile culture tubes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves