PFORCEKB

KAPA PROBE FORCE

2 ×, Universal

• Synonym(s): qPCR
• NACRES: NA.55
• UNSPSC Code: 41106300

Properties

• usage: sufficient for 100 reactions, 20 μL sufficient for 100 reactions, sufficient for 500 reactions, 20 μL sufficient for 500 reactions
• shelf life: ≤12 mo.
• feature: dNTPs included, hotstart
• packaging: kit of 1 mL (100 x 20 μL rxn; KK4300), kit of 5 mL (500 x 20 μL rxn; KK4301)
• manufacturer/tradename: Roche
• concentration: 2 ×
• technique(s): qPCR: suitable
• input: crude DNA
• detection method: probe-based
• storage temp.: −20°C

Description

General description

KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.

• Direct qPCR from crude blood, tissue, and plant extracts
• Sample-to-Cq workflows in <1 hour
• High efficiency for accurate, reproducible, and sensitive results
• Superior tolerance to carry-over inhibitors
• Multiplex compatibility with crude extracts

Application

KAPA PROBE FORCE has been used for:

• GMO testing
• Mouse transgenics
• SNP genotyping
• Food/water pathogen detection
• Infectious disease research
• Cancer research
• DNA quantification
• Quantitative polymerase chain reaction (qPCR)[1]
• Digital droplet PCR[2]
• For amplification of templates directly from cDNA synthesis reactions

Features and Benefits

Streamline sample-to-Cq workflows in <1 hour:

• Eliminate the time and cost of sample purification by amplifying directly from crude samples
• Analyze a wide range of sample types including whole blood, cells, mouse tails, FFPE, leaf, stem, seed, and soil

Generate accurate and reproducible results:

• Kits include a third-generation DNA polymerase, evolved for robust target amplification and detection
• Enzyme maintains high reaction efficiency in the presence of PCR inhibitors for reliable data generation

Break through high levels of qPCR inhibitors:

• Achieve greater levels of sensitivity for inhibited blood, tissue, and plant samples
• Convert purified DNA assays to crude workflows without observable Cq delays

Multiplex crude samples efficiently:

• Accelerate genotyping analysis with single reaction allelic discrimination of crude DNA extracts
• Maximize data collection from precious samples, increase throughput, and reduce costs

Quick Notes:

• This kit contains the KAPA3G HotStart DNA Polymerase enzyme, enabling probe-based qPCR for both routine and challenging sample types.
• Initial denaturation of 3 min at 98°C is recommended to ensure complete denaturation of complex target DNA. A 5-min denaturation time may be required for some crude samples.
• For two-step cycling, use a 20-sec combined annealing/extension/data acquisition at 60°C as a first approach.
• A 10-sec annealing/extension/data acquisition time may be used with most assays, but this must be determined empirically.
• For crude samples, the amount of sample in the reaction may be reduced to improve performance, but this must be determined empirically.

Preparation Note

ROX reference dye is sensitive to light exposure. Always ensure that the product has been fully thawed and mixed before use. Avoid repeated freezing and thawing.

Analysis Note

KAPA PROBE FORCE qPCR Master Mix Universal is subjected to stringent functional quality control, free of detectable contaminating exo- and endonuclease activities, and meets strict requirements with respect to DNA contamination.

Other Notes

For Research Use Only. Not for use in diagnostic procedures.

Kit Components Only

• KAPA3G HotStart^® DNA Polymerase
• dNTPs (including dUTP)
• MgCl₂ 4.5 mM at 1X
• ROX^™ reference dye
• stabilizers

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash