SCC118
CWR-R1ca Human Prostate Cancer Cell Line
Human
Synonym(s):
Fibroblast-Free CWR-R1 cell line
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About This Item
UNSPSC Code:
41106514
eCl@ss:
32011203
NACRES:
NA.81
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product name
CWR-R1ca Human Prostate Cancer Cell Line, CWR-R1ca Human Prostate Cancer Cell Line is a valuable cell model for castration-recurrent prostate cancer studies.
biological source
human
Quality Level
shipped in
ambient
General description
CWR-R1ca is a fibroblast-free cell line derived from the castration-resistant or recurrent CWR-R1 human prostate cancer cell line (1). Removal of fibroblasts from the original parental CWR-R1 cells was accomplished by multiple cycles of short-term trypsinization, cloning and pooling single-cell colonies (1). CWR-R1ca cells express AR-FL, and its splice variant AR-V7, CK-8, CK-18 and c-Met and are highly responsive to androgen for growth (1).
Key Applications:
• Androgen Receptor Splice Variant 7 (AR-V7) studies
• Anti-androgen therapy
• In vivo tumorigenesis
Key Applications:
• Androgen Receptor Splice Variant 7 (AR-V7) studies
• Anti-androgen therapy
• In vivo tumorigenesis
Prostate cancer is a leading cause of male cancer mortality. The androgen receptor (AR) is important for normal development and maintenance of the prostate and is also critical for the survival and progression of prostate cancer. Current treatment of advanced or metastatic prostate cancer relies on androgen deprivation by inhibiting androgen synthesis or anti-androgens that bind to the AR ligand binding domain. The initial response to androgen deprivation therapy is favorable, however, most patients inevitably relapse to a more aggressive, castration-resistant prostate cancer (CRPC), caused by continued transactivation of AR.
CWR-R1ca was derived from the castration-resistant or recurrent CWR-R1 human prostate cancer cell line . The original parental CWR-R1 is a human prostate carcinoma epithelial cell line derived from the recurrent CWR22 human xenograft tumors that were harvested from nude mice 140-160 days after castration and express AR full length (AR-FL), AR-V7 and PSA mRNA and protein .
CWR-R1ca was derived from the castration-resistant or recurrent CWR-R1 human prostate cancer cell line . The original parental CWR-R1 is a human prostate carcinoma epithelial cell line derived from the recurrent CWR22 human xenograft tumors that were harvested from nude mice 140-160 days after castration and express AR full length (AR-FL), AR-V7 and PSA mRNA and protein .
Cell Line Description
Cancer Cells
Application
Research Category
Cancer
Oncology
Cancer
Oncology
This product is intended for sale and sold solely to academic institutions for internal academic research use per the terms of the “Academic Use Agreement” as detailed in the product documentation. For information regarding any other use, please contact licensing@emdmillipore.com.
Quality
• Each vial contains ≥ 1X106 viable cells.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
• Cells are tested by PCR and are negative for HPV-16, HPV-18, Hepatitis A, B, C and HIV-1 & 2 viruses.
• Cells are negative for mycoplasma contamination.
• Each lot of cells are genotyped by STR analysis to verify the unique identity of the cell line.
Storage and Stability
Store in liquid nitrogen. The cells can be cultured for at least 10 passages after initial thawing without significantly affecting the cell marker expression and functionality.
Disclaimer
RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M Nagabhushan et al.
Cancer research, 56(13), 3042-3046 (1996-07-01)
Most patients' prostate cancers respond to androgen deprivation but relapse after periods of several months to years. Only two prostate cancer xenografts, LNCaP and PC-346, have been reported to be responsive to androgen deprivation and to relapse subsequently. Both of
Mojgan Shourideh et al.
The Prostate, 76(12), 1067-1077 (2016-06-09)
The previously established CWR-R1 cell line has been used as an in vitro model representing castration-recurrent prostate cancer. Microscopic observation of subconfluent cells demonstrated two distinct cellular morphologies: polygonal closely aggregated epithelial cells surrounded by bipolar fibroblastic cells with long
C W Gregory et al.
Cancer research, 61(7), 2892-2898 (2001-04-18)
The androgen receptor (AR) is highly expressed in androgen-dependent and recurrent prostate cancer (CaP) suggesting it has a role in the growth and progression of CaP. Previously proposed mechanisms for AR reactivation in recurrent CaP include altered growth factor signaling
Rintaro Hashizume et al.
Journal of neuro-oncology, 110(3), 305-313 (2012-09-18)
Diffuse intrinsic pontine gliomas arise almost exclusively in children, and despite advances in treatment, the majority of patients die within 2 years after initial diagnosis. Because of their infiltrative nature and anatomic location in an eloquent area of the brain
Andras Franko et al.
Genes, 11(10) (2020-10-11)
Prostate cancer (PCa), the most incident cancer in men, is tightly regulated by endocrine signals. A number of different PCa cell lines are commonly used for in vitro experiments, but these are of diverse origin, and have very different cell-proliferation
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